小分子化合物Neostigmine Bromide抑制肺癌细胞增殖及其分子机制

The effect and mechanism of Neostigmine Bromide surpressing the proliferation of lung cancer cells

目的研究小分子化合物Neostigmine Bromide对人肺癌细胞增殖、迁移能力的影响以及其分子机制。方法分别用0、10、20、40μmol/L浓度的Neostigmine Bromide处理A549、NCI-H460、NCI-H1299三种肺癌细胞,用CCK8检测不同浓度小分子化合物Neostigmine Bromide对肺癌细胞A549、NCI-H460、NCI-H1299增殖能力的影响。利用克隆形成实验验证化合物对肺癌细胞A549、NCI-H460增殖能力的抑制作用。应用Transwell实验检测该药物对A549和NCI-H1299细胞迁移能力的影响。Western blot技术检测化合物对NCI-H460细胞周期调控蛋白Cyclin D1和CDK4表达含量的影响。结果化合物Neostigmine Bromide通过剂量依赖的方式抑制人肺癌细胞A549、NCI-H460、NCI-H1299的增殖能力,在一定程度上抑制肺癌细胞的迁移能力,并以剂量依赖的方式下调肺癌细胞NCI-H460中Cyclin D1和CDK4的蛋白表达水平。结论 Neostigmine Bromide抑制肺癌细胞增殖和迁移能力与下调Cyclin D1和CDK4的表达水平相关。

Objective To study the effect and mechanism of Neostigmine Bromide on the proliferation and migration of human lung cancer cells. Methods CCK8 method was used to determine the proliferation of A549, NCI-H460 and NCI-H1299 cells. The inhibitory effect of compound Neostigmine Bromide on the proliferation of tumor cells A549, NCI-H460 was performed by Clone-formation. The Transwell was used to check the migration of A549 and NCI-H1299 cells. The expression of Cyclin D1 and CDK4 was detected by Western blot. Results Neostigmine Bromide suppressed the proliferation of A549, NCI-H460, NCI-H1299 cells in a dose-dependent manner with ability which inhibit the migration of the lung cancer cells to a certain extent. Neostigmine Bromide significantly down-regulated the expression levels of Cyclin D1 and CDK4 in NCI-H460 cells. Conclusion The inhibition effect of Neostigmine Bromide on human lung cancer cells is related to the downregulation of Cyclin D1 and CDK4 expression.