尾矿藻表面磷调控及其强化固定重金属研究

Research on the Regulation of Phosphorus on the Surface of Tailing Algae and Its Ability to Strengthen Immobilizing Metals

从铅锌尾矿废弃地中分离纯化获得一株高重金属离子耐受性微藻Didymogenes palatina XR,通过改变培养基中磷浓度调控细胞的表面含磷官能团,获得高载磷微藻,然后探究影响微藻固定重金属铅的能力及机理。研究结果表明:在最优pH为6时,高载磷活藻对金属离子铅的去除率最高,达91.59%;在160 mg/L的磷浓度培养改性的条件下,活藻细胞对Pb2+的最大吸附量最高,达7.93 mg/g;该磷改性条件下,藻细胞表面磷基官能团和总官能团含量最高,且FT-IR分析表明含磷基团主导微藻对重金属离子的吸附。本研究表明不同磷浓度培养能调控微藻表面载磷量并强化其对重金属的固定能力。

Didymogenes palatinaXR,a microalga with high tolerance of heavy metal ions,was isolated and purified from an abandoned mine of lead and zinc tailings. The high-phosphorous microalga was obtained by changing the phosphorus concentration in the culture medium. Regulate the surface phosphorous functional groups of the cells to investigate the ability and mechanism of the microalga immobilizing the lead. The results showed the optimal pH was 6,when the removal rate of lead ions was the highest reaching to 91.59%. Under the condition of 160 mg/L phosphorus concentration culture modification,the maximum adsorption of Pb2+by living algae cells was the highest,up to 7.93 mg/g,and the content of phosphorus and total functional groups on the surface of algal cells was the highest,and FT-IR analysis indicated that phosphorus groups on the surface of cells dominated the adsorption of heavy metal ions by microalgae. This study showed that different phosphorus concentrations could regulate the phosphorus load on the surface of microalgae and enhance their ability to fix metals.