蒙药止泻木子、连翘、地梢瓜对腹泻大鼠止泻作用比较研究

A comparative study on antidiarrheal effects of Mongolian medicines Holarrhena antidysenterica,Forsythia suspensa and Cynanchum thesioides

目的比较蒙药止泻木子Holarrhena antidysenterica、连翘Forsythia suspensa、地梢瓜Cynanchum thesioides对腹泻模型大鼠止泻作用及血清二胺氧化酶(DAO)、环磷酸腺苷(cAMP)、肿瘤坏死因子-α(TNF-α)、ATP酶、钙离子的影响。方法设正常对照组,模型组,止泻木子低、高剂量组,连翘低、高剂量组,地梢瓜低、高剂量组,除正常组外其他组ig给药番泻叶水煎剂建立腹泻模型,模型成功后ig药物7 d,观察各组小鼠精神状态、腹泻情况及体质量变化,最后1 d给药后禁食12 h腹主动脉取血,使用酶联免疫检测试剂(ELISA)试剂盒检测血清中DAO、cAMP、TNF-α、ATP酶水平及Ca2+质量浓度。结果一般状态:除正常组外其他组大鼠造模后精神状态萎靡,皮毛色泽明显下降,体质量下降,造模后第4天腹泻率为100%,给药第4天开始各给药组大鼠体质量和精神状态明显恢复,以地梢瓜高剂量组大鼠体质量上升最明显;稀便数、稀便级及腹泻指数,与模型组比较,各给药组均有显著差异(P<0.05、0.01);与模型组比较,止泻木子低剂量组、地梢瓜低剂量组和连翘高、低剂量组大鼠血清DAO水平明显降低,有显著差异(P<0.05);与模型组比较,地梢瓜高、低剂量组大鼠血清TNF-α水平明显降低有显著差异(P<0.05);与模型组比较,地梢瓜高、低剂量组血清ATP酶浓度降低,有显著差异(P<0.05);与模型组比较,止泻木子高剂量组和连翘低剂量组血清cAMP浓度明显降低,有显著差异(P<0.05);与模型组比较,各给药组血清Ca2+质量浓度均增高,有显著差异(P<0.05)。结论地梢瓜对番泻叶所致大鼠腹泻模型的止泻作用强于止泻木子和连翘;但3种药材对大鼠血清中细胞因子和离子的抑制作用不同,地梢瓜以对TNF-α和ATP酶的抑制作用为主,止泻木子以对cAMP和钙离子有调控作用为主,连翘以对DAO抑制作用为主,因而,3种药材在作用机制方面存在一定差异。

Objective To compare the antidiarrheal effects of Mongolian medicine,Holarrhena antidysenterica,Forsythia suspensa and Cynanchum thesioides on diarrhea model rats and investigate its effects on serum DAO(diamine oxidase),cAMP(cyclic adenosine phosphate),TNF-α(tumor necrosis factor-α),ATPase and calcium ions.Methods The normal control group,model group,H.antidysenterica low-dose and high-dose groups,F.suspensa low dose and high-dose groups,C.thesioides low dose and high dose groups were set.Except the normal control group,the other groups were ig administrated water decoction of Cassia angustifolia to establish diarrhea model;After the success of the model,the rats in treatment groups were administrated by gastric drug for 7 d,the type mental state,diarrhea and body weight changes were observed.the abdominal aortic blood was obtained at the last day of fasting 12 h after the administration.DAO,cAMP,TNF-α,ATP enzyme,OD value of the calcium ions in serum were determined by using ELISA(enzyme-linked immune detection reagent)kits.Results General status:except for the normal group,the mental state of the rats in the other groups was depressed after modeling,the fur color of them was significantly decreased and the body weight was decreased.The diarrhea rate was 100%on the 4th day after modeling.Compared with the model group,there were significant differences in the number of loose stools,grade of loose stools and diarrhea index(P<0.05,0.01)in each administration group.The serum DAO,TNF-α,ATPase,cAMP and calcium ion OD values were compared:the serum concentrations of DAO in H.antidysenterica low dose group,C.thesioides low dose group and F.suspensa high and low dose groups of rats were significantly lower than model group with significant differences(P<0.05),and were significantly higher than normal group.The serum concentration of TNF-αin C.thesioides high and low dose groups were lower compared with model group(P<0.05).The serum ATPase in C.thesioides high and low dose groups had significant difference(P<0.05)compared with model group.The serum concentration of cAMP in H.antidysenterica high-dose group and F.suspensa low-dose group was significantly lower compared with model group with significant differences(P<0.05).Serum Ca2+concentration in the drug administration groups was significantly different from that in the model group(P<0.05).Conclusion The antidiarrheal effect of C.thesioides is better than that of H.antidysenterica and F.suspensa.