醇型和酮型广藿香DNA甲基化的MSAP分析

MSAP analysis of DNA methylation between pogoston-type and patchouliol-type of Pogostemon cablin

目的使用MSAP技术对2种化学型广藿香Pogostemon cablin的基因组DNA进行甲基化分析。方法使用甲基化敏感扩增多态性(methylation sensitive amplification polymorphism,MSAP)技术检测2种化学型、共计197个广藿香样本的DNA甲基化程度。结果4类MSAP位点信息的Shannon多态性指数的最高值或较高值均在阳春、德庆、高州大垌、禄步、广宁5个产地中产生;石牌广藿香(酮型)与其余产地的广藿香(醇型)形成2个分支;广藿香居群间的变异所占百分比远大于居群内变异所占百分比,达60.66%;共筛选出10条石牌产地与其余产地的差异性片段并进行测序分析,其中一条属于丝氨酸/苏氨酸蛋白激酶家族。结论可通过MSAP技术在不同来源的样品中鉴别出石牌产地的广藿香;广藿香不同化学型的形成与其DNA甲基化水平有着密切的联系,尚需更进一步的研究。

Objective To analyze the DNA methylations between two chemical types of Pogostemon cablin using MSAP technique.Methods Methylation sensitive amplification polymorphism(MSAP)technique was used to detect the DNA methylation degree of two chemical type and a total of 197 P.cablin samples.Results The highest or higher values of the Shannon polymorphism index of the four types of MSAP locus information were produced in the five areas of Yangchun,Deqing,Gaozhou Datong,Lubu,and Guangning.It formed two branches with P.cablin Shipaiensis(Pogostone-type)and the rest(Patchouliol-type).The percentage of variation among the populations was 60.66%,which was far greater than that within the population.A total of 10 differential fragments were screened out,sequenced and analyzed,one of those belongs to the serine/threonine protein kinase family.Conclusion The MSAP technique can be used to identify the different origins of P.cablin.The formation of different chemical types of P.cablinis closely related to its DNA methylation level,and further research is needed.