基于网络药理学的参芪降糖颗粒治疗2型糖尿病机制探讨

Mechanism of Shenqi Jiangtang Granules in treatment of type 2 diabetes based on network pharmacology

目的运用网络药理学的方法探讨参芪降糖颗粒治疗2型糖尿病的作用机制。方法通过文献挖掘和数据库检索获取参芪降糖颗粒的活性成分、作用靶点及与2型糖尿病相关的疾病靶点,通过Enrichr数据库进行基因本体论(GO)分析和京都基因与基因组百科全书(KEGG)通路分析,并运用Cytoscape3.5.1软件绘制网络互作图,imageGP工具绘制GO和KEGG气泡图。结果研究得到43个关键活性成分和30个关键靶点,GO富集分析有918个条目,包含细胞组分(CC)条目56个、分子功能(MF)条目99个和生物学进程(BP)条目763个,其中CC主要涉及轴突轴膜、核转录因子复合物、RNA聚合酶II转录因子复合物、核常染色质、血小板α颗粒;MF主要涉及MAP激酶活性、血管内皮生长因子受体结合等方面;BP主要涉及血管生成、泡沫细胞分化、白细胞介素-21的细胞反应等方面。KEGG通路富集分析包含KEGG通路105条,主要与IL-17信号通路、糖尿病并发症的AGE-RAGE信号通路、VEGF信号通路、线粒体自噬等信号通路有着密切联系。结论参芪降糖颗粒可能通过作用于神经内分泌失调、胰岛素抵抗、氧化应激、炎症等相关的靶点及通路,起到治疗2型糖尿病的作用。

Objective To explore the mechanism of Shenqi Jiangtang Granules in treatment of type 2 diabetes mellitus by network pharmacology. Methods The active components and targets of Shenqi Jiangtang Granules and targets associated with type 2 diabetes were obtained by literature mining and database. Enrichr database was used to analyze gene ontology enrichment and Kyoto gene and genome encyclopedia. In addition, Cytoscape 3.5.1 software was used to draw network interaction diagrams, and imageGP tool was used to draw GO and KEGG bubble diagrams. Results A total of 43 key active ingredients and 30 key targets were obtained. GO enrichment analysis included 918 items, including 56 cell component(CC) entries, 99 molecular function(MF) entries, and 763 biological process(BP) entries. CC entries were related to axonal membrane, nuclear transcription factor complex, RNA polymerase Ⅱ transcription factor complex, nuclear euchromatin, and platelet α granule. MF entries were mainly related to MAP kinase activity, vascular endothelial growth factor receptor binding and other aspects. BP entries were related to biological processes, mainly including angiogenesis, foam cell differentiation, and cellular response to interleukin-21. The KEGG pathway enrichment analysis included 105 KEGG pathways, which were closely related to IL-17 signaling pathway, AGE-RAGE signaling pathway in diabetic complications, VEGF signaling pathway, mitophagy and other signaling pathways. Conclusion Shenqi Jiangtang Granules may play a role in treatment of type 2 diabetes by acting on neuroendocrine disorders, insulin resistance, oxidative stress, inflammation, and other related protein targets and pathways.