P75NTR、SOX2在成釉细胞瘤中的表达及意义

Expression and clinical significance of P75NTR,SOX2 in ameloblastoma

目的检测干细胞标记物P75NTR、SOX2在成釉细胞瘤(ameloblastoma,AM)中的表达情况,探讨AM中是否存在肿瘤干细胞及肿瘤干细胞与其生物学行为的相关关系。方法选择28例不同分型的AM病例为研究对象;另取5例含牙囊肿、5例牙源性角化囊肿作对照。用免疫组化染色技术检测P75NTR、SOX2的表达情况,用非参数秩和检验检测P75NTR和SOX2在实性型AM和单囊型AM中的表达差异、AM与含牙囊肿及牙源性角化囊肿之间的表达差异。结果P75NTR在实性型AM表达强度明显高于单囊型AM;在AM的表达强度明显高于含牙囊肿及牙源性角化囊肿,差异具有统计学意义(P<0.05);SOX2在实性型与单囊型AM、AM与含牙囊肿之间的表达无明显差异(P>0.05),AM与牙源性角化囊肿之间表达有差异(P<0.05)。结论在AM中有干细胞标志物P75NTR的表达,可能与细胞增殖及干细胞特性相关,并参与肿瘤侵袭性。SOX2在AM中表达较少,其在AM中的作用有待于进一步探讨。

Objective To detect the expression of stem cell markers P75 NTR and SOX2 in ameloblastoma(AM),and to investigate the correlation between tumor stem cells and their biological behavior.Methods Twenty-eight AM cases with different types were selected as subjects,and 5 cases of odontogenic cysts and 5 cases of keratocyst were taken as control group.Immunohistochemical staining was used to detect the expression of P75 NTR and SOX2,and non-parametric rank-sum test was used to analyze the expression difference of P75 NTR and SOX2 between solid AM and unicystic AM,as well as the expression difference between AM and dentigerous cyst and keratocyst.Results The expression intensity of P75 NTR in solid AM was significantly higher than that in unicystic AM.The expression intensity of P75 NTR in AM was significantly higher than that in dentigerous cyst and keratocyst(P<0.05).These differences showed a statistical meaning.There was no significant difference in SOX2 expression between solid AM and unicystic AM,AM and odontogenic cyst(P>0.05),and there was difference between AM and keratocyst(P<0.05).Conclusion The expression of the stem cell marker P75 NTR in AM may be related to cell proliferation and stem cell characteristics,and may be involved in tumor invasiveness.SOX2 is less expressed in AM,and its role in AM remains to be further explored.