肉桂醛对口腔鳞状细胞癌细胞CAL27增殖和迁移的影响

Effect of cinnamaldehyde on the proliferation and migration of oral squamous cell carcinoma CAL27 cells

目的探讨不同浓度肉桂醛在体外二维条件下对口腔鳞状细胞癌(简称鳞癌)细胞CAL27的增殖、迁移、侵袭、凋亡和上皮间充质转化的影响。方法通过CCK8实验检测肉桂醛对口腔鳞癌细胞CAL27增殖性的抑制作用;通过划痕实验检测肉桂醛对CAL27的迁移能力是否具有抑制作用;通过transwell小室侵袭实验检测肉桂醛对CAL27的侵袭能力是否具有抑制作用;通过流式细胞术检测肉桂醛对CAL27的凋亡是否具有促进作用;通过Western blot实验检测肉桂醛对CAL27的上皮间充质转化是否产生影响。结果CCK8显示,经肉桂醛处理的实验组呈作用时间和药物浓度依赖性抑制CAL27细胞的增殖;细胞划痕和transwell小室侵袭实验表明,与对照组相比,肉桂醛处理组能够呈剂量依赖性抑制细胞迁移和侵袭能力;流式细胞术显示,肉桂醛呈剂量依赖性促进细胞CAL27凋亡;Western blot表明,肉桂醛呈剂量依赖性抑制CAL27细胞Vimentin、Snail表达,促进E-cadherin表达。结论经肉桂醛处理的实验组在体外二维条件下呈剂量依赖性抑制口腔鳞癌细胞CAL27的增殖、迁移和侵袭并促进其凋亡。肉桂醛通过影响上皮间充质转化过程从而对CAL27的迁移能力产生抑制作用。

Objective To study the effect of different concentrations of cinnamaldehyde on the proliferation,migration,invasion,apoptosis and epithelial-mesenchymal transition of oral squamous cell carcinoma(OSCC)CAL27 cells in two dimensional conditions in vitro.Methods CCK8 test was performed to determine whether cinnamaldehyde can inhibit the proliferation of OSCC CAL27.Scratch test was conducted to determine whether cinnamaldehyde has an inhibitory effect on the migration of CAL27.Transwell test was used to test whether cinnamaldehyde can inhibit the invasion of CAL27.Flow cytometry was used to test whether cinnamaldehyde can promote the apoptosis of CAL27.Western blot was performed to determine whether cinnamaldehyde has an effect on the epithelial-mesenchymal transition of CAL27.Results The results of CCK8 showed that the experimental group treated with cinnamaldehyde had a time and drug concentration dependence effect on inhibiting the proliferation of CAL27.The results of scratch test and transwell test showed that the experimental group treated with cinnamaldehyde inhibited the cell migration ability and invasion ability in a dose-dependent manner.The results of flow cytometry showed that the experimental group treated with cinnamaldehyde promoted the apoptosis of CAL27 in a dose-dependent manner.The results of Western blot showed that the experimental group treated with cinnamaldehyde inhibited the expression of Vimentin and Snail and promoted the expression of E-cadherin in a dose-dependent manner.Conclusion The experimental group treated with cinnamaldehyde inhibits the proliferation,migration and invasion of OSCC CAL27 and promotes its apoptosis in a dose-dependent manner in two dimensional conditions in vitro.Cinnamaldehyde inhibits the migration of OSCC CAL27 by affecting the epithelial-mesenchymal transition process.