黑龙江萝北口岸蜱传立克次体分子流行特征研究

Molecular epidemiological characterization of tick-borne Rickettsia at Luobei port,Heilongjiang province

目的了解我国和俄罗斯边境媒介蜱携带立克次体携带情况及其分子流行特征。方法2019年在黑龙江省萝北口岸地区采集蜱,经形态学鉴定后提取DNA,用PCR方法检测立克次属核酸片段,对阳性样本进行测序,利用Mega6.06、Lasergene软件进行序列分析,构建系统进化树。结果共采集蜱63只,其中61只为森林革蜱,2只为全沟硬蜱。1只森林革蜱检测到立克次体核酸,检出率为1.59%。经基因序列同源性分析,为斑疹伤寒群立克次体—劳氏立克次体,显示与黑龙江株(MG906669)同源性最高。结论萝北口岸地区存在立克次体传播风险。

Objective To investigate the prevalence of tick-borne Rickettsia at the border area between China and Russia.Methods The ticks were collected in Luobei port area of Heilongjiang province in 2019.DNA was extracted after morphological identification.The nucleic acid of Rickettsia was detected by PCR.The positive samples were sequenced and analyzed by using Mega 6.06 and Lasergene software to construct phylogenetic tree.Results A total of 63 ticks were collected,of which 61 were Dermacentor silvarum and 2 were Ixodes persuleatus.Rickettsia nucleic acid was detected in one Dermacentor silvarum with a detection rate of 1.59%.Through the homology analysis of gene sequence,it was Rickettsia raoultii,which showed the highest homology with Heilongjiang strain(MG906669).Conclusion There is risk of Rickettsia transmission in Luobei port area.