中国广东地区膝骨关节炎患者与健康人群肠道菌群差异性分析

Analysis on the difference of intestinal flora between patients with knee osteoarthritis and healthy people in Guangdong province

目的比较中国广东地区骨关节炎(osteoarthritis,OA)患者与健康人群肠道菌群的宏基因组,初步分析其差异性及差异特征。方法选取我院从2017年6~12月收集的29名健康人群,66例膝OA患者粪便样本,其中膝OA患者根据K-L分型分为轻度OA组17例,重度OA组49例。检测前进行对基线比较。样本提取DNA后,使用Simpson多样性指数分析群落多样性,使用LEfSe分析对群落关键物种进行筛选;差异物种GO分析及Pathway分析进行差异性菌群代谢功能预测。结果3组在身高、年龄及体重上没明显差异(P>0.05);Simpson多样性指数分析表明轻度OA患者与健康人群肠道菌群多样性无显著差异,重度OA患者肠道微生物多样性显著低于健康人群。LEfSe分析发现,寡养单胞菌属(Stenotrphomonas)、黄单胞菌属(Xanthomonadacease)等18种菌群在膝骨关节炎患者人群中显著高丰度,毛螺旋菌属(Lachnospiraceae)、瘤胃球菌属(Ruminococcus)等6种菌群在膝骨关节炎患者人群中显著低丰度。寡养单胞菌属、黄单胞菌属两类菌群可以显著区分轻重型膝骨关节炎。差异物种GO分析提示寡养单胞菌属、黄单胞菌属等菌群的显著升高,对代谢类疾病、感染性疾病、心血管类疾病、免疫性疾病的发病具有增加的趋势。结论广东地区膝骨关节炎患者肠道菌群丰度和某些菌群与健康人群有显著差异,寡养单胞菌属、黄单胞菌属两类菌群可以显著区分轻重型膝骨关节炎。

Objective To compare the intestinal flora metagenomics between osteoarthritis(OA)patients and healthy people in Guangdong province,and to preliminarily analyze the difference and specialty of both groups.Methods Fecal samples were collected from 29 healthy people and 66 patients with knee OA from June 2017 to December 2017 in the Hospital of TCM in Guangdong province.Among them,17 patients with knee OA were classified into mild OA group and 49 patients were classified into severe OA group,according to K-L classification.Baseline comparison was performed prior to the test.After DNA extraction,Simpson diversity index was used to analyze the community diversity and LEfSe analysis was used to screen key species in the community.GO analysis and Pathway analysis were used to predict the metabolic function of the different flora.Results There was no significant difference in height,age,and weight among the three groups(P>0.05).Simpson diversity index analysis showed that there was no significant difference in intestinal microbial diversity between patients with mild OA and healthy people,while intestinal microbial diversity of patients with severe OA was significantly lower than that of healthy people.LEfSe result showed that 18 species of bacteria,such as Stenotrphomonas and Xanthomonadacease,were significantly abundant in patients with knee OA,while 6 species of bacteria,including Lachnospiraceae and Ruminococcus,were significantly abundant in patients with knee OA.Stenotrphomonas and Xanthomonadacease significantly distinguished patients with mild or heavy knee OA.The GO analysis of the differential species showed that the bacterial flora of Stenotrphomonas and Xanthomonadacease increased significantly,indicating an increasing tendency of the incidence of metabolic diseases,infectious diseases,cardiovascular diseases,and immune diseases.Conclusion There are significant differences in the abundance of intestinal microflora and some microflora between the patients with knee OA and healthy people in Guangdong province.Stenotrphomonas and Xanthomonadacease can be used to obviously predict or diagnosis mild and severe knee OA.