白藜芦醇对脑组织缺血再灌注大鼠肿瘤坏死因子-α、白介素-6、基质金属蛋白酶9及白介素-1β水平影响

Effects of resveratrol on levels of tumor necrosis factor-a,interleukin-6,metalloproteinase-9 and interleukin-1βin cerebral ischemia-reperfusion rats

目的探讨白藜芦醇(Res)对脑组织缺血再灌注损伤的影响,以及其对炎症因子表达水平的影响。方法选取健康成年SPF级SD大鼠30只,体重200~220 g,按随机数表法分为三组:假手术组(Sham组)、脑组织缺血再灌注组(MI/R组)、脑组织缺血再灌注+白藜芦醇组(MI/R+Res组)。采用改良线栓法建立大鼠大脑中动脉栓塞模型,建模前连续7 d予MI/R+Res组腹腔注射Res 30 mg/kg,余两组腹腔注射等剂量的0.9%氯化钠溶液。采用酶联免疫吸附法(ELISA)检测三组血清中肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、基质金属蛋白酶9(MMP-9)及白介素-1β(IL-1β)的水平;提取缺血脑组织,通过逆转录-聚合酶链式反应(RT-PCR)测TNF-α、IL-6、MMP-9 mRNA的水平。结果与Sham组相比,MI/R组及MI/R+Res组大鼠血清炎症因子TNF-α、IL-6、MMP-9及IL-1β的水平明显增加(P <0.05);与MI/R组相比,MI/R+Res组TNF-α、IL-6、MMP-9及IL-1β的水平显著减少(P <0.05);与Sham组相比,MI/R组及MI/R+Res组缺血脑组织TNF-α、IL-6、MMP-9 mRNA的表达明显增加(P <0.05);与MI/R组相比,MI/R+Res组TNF-α、IL-6、MMP-9 mRNA的表达显著降低(P <0.05)。结论 Res可以降低脑组织缺血再灌注大鼠炎症因子的表达水平,减轻炎症反应。

Objective To investigate the effects of resveratrol on cerebral ischemia reperfusion injury and the expression of inflammatory factors.Methods Thirty healthy adult SPF SD rats,weighing 200-220 g,were randomly divided into three groups:sham operation group(sham group),cerebral ischemia reperfusion group(MI/R group)and cerebral ischemia reperfusion and resveratrol group(MI/R+Res group).The model of middle cerebral artery embolism in rats was established by modified suture embolization method.The MI/R+Res group were intraperitoneally injected with resveratrol 30 mg/kg for 7 days before the establishment,and the other two groups were injected with saline 30 mg/kg for 7 days.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of tumor necrosis factor-a(TNF-α),interleukin-6(IL-6),metalloproteinase-9(MMP-9)and interleukin-1β(IL-1β)in serum.Ischemic brain tissues were extracted and the contents of TNF-α,IL-6 and MMP-9 mRNA were measured by reverse transcription-polymerase chain reaction(RT-PCR).Results Compared with sham group,the levels of TNF-α,IL-6,MMP-9 and IL-1βincreased significantly in MI/R group and MI/R+Res group(P<0.05).Compared with MI/R group,the levels of TNF-α,IL-6,MMP-9 and IL-1βdecreased significantly in MI/R+Res group(P<0.05).Compared with sham group,the expressions of TNF-α,IL-6 and MMP-9 mRNA increased in MI/R group and MI/R+Res group(P<0.05).Compared with MI/R group,TNF-α,IL-6 and MMP-9 mRNA expressions decreased significantly in MI/R+Res group(P<0.05).Conclusion Resveratrol can decrease the expression of inflammatory factors and alleviate inflammation in rats with cerebral ischemia reperfusion injury.