白藜芦醇改善γ射线诱导的T-淋巴细胞损伤大鼠的免疫功能

Resveratrol Improved the Immune Function of Rats Exposed to T-lymphocyte Injury Induced by Samray

本文研究了白藜芦醇改善γ射线诱导的T-淋巴细胞损伤大鼠的免疫功能。选取SPF级50只SD健康大鼠,随机分为空白组、模型组、低剂量组(20μmol/L)、中剂量组(40μmol/L)和高剂量组(80μmol/L),建立γ射线诱导的T淋巴细胞损伤模型大鼠。建模成功后,对大鼠进行白藜芦醇灌胃处理,按实验设计对大鼠细胞T细胞增殖率、T淋巴细胞核因子-kB(Nuclear factor kB,NF-kB)表达、T淋巴细胞(CD3、CD4、CD8)、干扰素-γ(Interferon-γ,IF-γ)、白介素-12(Interleukin-12,IL-12)、白介素-4(Interleukin-4,IL-4)、白介素-3(Interleukin-3,IL-3)水平进行检测。结果表明,中剂量组(40μmol/L)白藜芦醇的T细胞增殖率、CD3、CD4、CD8、CD4/CD8分别为2.15%、54.15%、35.11%、21.45%、2.11。白藜芦醇对T细胞分泌的IF-γ、IL-12、IL-4、IL-3的水平分别为21546.15 pg/mL、212.15 pg/mL、645.56 pg/mL、221.15 pg/mL,与其他实验组均呈显著差异(p<0.05),说明白藜芦醇能够明显抑制调节T细胞,改善大鼠的免疫功能。

To study the effect of resveratrol on immune function of rats exposed to t-lymphocyte injury induced by tpa,50 SPF-level SD healthy rats were randomly divided into blank group,model group,low-dose group(20μmol/L),medium-dose group(40μmol/L)and high-dose group(80μmol/L),to establish the tlymphocyte injury model rats induced byγ-ray.After successful modeling,rats were given resveratrol by gavage.According to the experimental design,the proliferation rate of T cells,nuclear factor kB of T lymphocytes(NF-kB)expression,level of T lymphocytes(CD3,CD4,CD8),interferon-γ(IF-γ),interleukin-12(IL-12),interleukin-4(IL-4),and interleukin-3(IL-3)were detected.The results showed that the proliferation rate,CD3,CD4,CD8 and CD4/CD8 of resveratrol group(40μmol/L)were 2.15%,54.15%,35.11%,21.45%and 2.11,respectively.The levels of IF-γ,IL-12,IL-4 and IL-3 secreted by T cells were 21546.15 pg/mL,212.15 pg/mL,645.56 pg/mL and 221.15 pg/mL,respectively,which were significantly different from those of other experimental groups(p<0.05).It is understood that resveratrol can significantly inhibit and regulate T cells and improve the immune function of rats.