Exendin-4对MCAO再灌注导致的脑缺血/再灌注损伤具有神经保护作用

Neuroprotective effect of Exendin-4 on cerebral ischemia/reperfusion injury induced by MCAO reperfusion

目的探讨GLP-1受体激动剂Exendin-4腹腔给药对大脑中动脉闭塞(MCAO)再灌注所致大鼠脑缺血/再灌注损伤的神经保护作用。方法SD大鼠术前1 h腹腔注射Exendin-4,MCAO再灌注24 h后进行神经功能缺损评分,TTC染色计算脑梗死体积,免疫荧光观察神经元和小胶质细胞生存数量及检测凋亡通路相关蛋白的相对表达水平。结果Exendin-4能够保护由于MCAO再灌注后所致的脑缺血再灌注损伤,减少了脑梗死体积,降低皮层凋亡蛋白的相对表达水平,抑制神经元凋亡。结论Exendin-4可以对MCAO再灌注所致脑缺血/再灌注损伤具有神经保护作用,该作用是通过抑制凋亡蛋白的产生,从而抑制细胞凋亡。

Objective To investigate the neuroprotective effect of exendin-4,a GLP-1 receptor agonist,on cerebral ischemia/reperfusion injury induced by middle cerebral artery occlusion(MCAO)reperfusion in rats.Methods SD rats were given exendin-4 intraperitoneally 1 h before operation,and MCAO reperfusion injury was operated.Neuroprotective effect was valued after 24 h of reperfusion through neurologic deficit score,infarct size,survival count of neuron and microglial.Infarct size was counted by TCC staining.Survival count of neuron and microglial cells were analyzed by immunofluorescence and the expression levels of correlative protein about apoptosis pathway were tested by western blot.Results Exendin-4 could protect the cerebral ischemia/reperfusion injury caused by MCAO reperfusion,reduce the infarct volume and the expression levels of apoptosis protein in cortex,and inhibit neuron apoptosis.Conclusion Exendin-4 had neuroprotective effect on cerebral ischemia/reperfusion injury induced by MCAO reperfusion,which could inhibit neuron apoptosis by inhibiting the production of apoptotic protein.