巨噬细胞迁移抑制因子基因多态性与颈动脉粥样硬化及斑块易损性的相关性研究

The relationship between macrophage migration inhibition factor gene polymorphism and carotid atherosclerosis,vulnerability of carotid plaque

目的探讨巨噬细胞迁移抑制因子(MIF)基因多态性与颈动脉粥样硬化及斑块易损性的关系。方法收集2018年1月-2019年12月本科收治475例缺血性脑卒中患者纳入研究,根据颈部血管超声表现分为无斑块组(n=107)、稳定斑块组(n=172)和易损斑块组(n=196);采用ELISA法检测所有入组人员血清MIF水平;采用聚合酶链反应联合DNA直接测序法检测MIF基因-173G/C位点基因型。结果稳定斑块组和易损斑块组血清MIF水平均显著高于无斑块组(P均<0.05),易损斑块组血清MIF水平显著高于稳定斑块组(P<0.05);稳定斑块组和易损斑块组MIF基因-173G/C位点基因型和等位基因频率与无斑块组比较均有明显差异(P均<0.05);稳定斑块组基因型和等位基因频率与易损斑块组比较均无明显差异(P均>0.05);CC基因型携带者血清MIF水平均显著高于GG和GC基因型携带者(P均<0.05)。校正年龄、性别等参数后-173G/C位点C等位基因携带者颈动脉斑块总体发生风险是T等位基因携带者的2.035倍(95%CI=1.284~3.227,P=0.003)。结论MIF基因-173G/C位点多态性与颈动脉硬化密切相关,携带C等位基因是颈动脉粥样硬化斑块形成的危险因素,但该位点基因多态性与颈动脉斑块易损性无明确相关性。

Objective To investigate the relationship between macrophage migration inhibition factor(MIF)gene polymorphism and carotid atherosclerosis,vulnerability of carotid plaque.Methods A total of 475 patients with ischemic stroke hospitalized in our department from January 2018 to December 2019 were recruited in this study.These patients were divided into non-plaque group(107 cases),stable plaque group(172 cases)and vulnerable plaque group(196 cases),according to the manifestations of carotid ultrasonography.ELISA was used to detect serum levels of MIF.Genotype was determined by polymerase chain reaction-direct sequencing for the MIF gene-173 G/C polymorphism.Results Serum levels of MIF in stable plaque group and vulnerable plaque group were higher than those in non-plaque group(both P<0.05).Serum level of MIF in vulnerable plaque group was higher than that in stable plaque group(P<0.05).The genotype and allele frequencies of-173 G/C in stable plaque group and vulnerable plaque group were significantly different from those in non-plaque group(all P<0.05).There was no significant difference in genotype and allele frequencies of-173 G/C between stable plaque group and vulnerable plaque group(both P>0.05).Serum MIF levels of CC genotype carriers were higher than those of CG and GG genotype carriers(both P<0.05).After adjusting risk factors(age,gender,etc),the risk of carotid atherosclerostic plaque in-173 G/C C allele carriers was 2.035 times of that in G allele(95%CI=1.284~3.227,P=0.003).Conclusion MIF-173 G/C gene polymorphism was associated with carotid atherosclerosis,and the-173 G/C C allele might act as a risk factor for carotid atherosclerosis plaque.However,-173 G/C gene polymorphism might not be associated with vulnerability of carotid plaque.