摘要
微流控芯片技术具有高度集成化、微型化等优势,是体外诊断(In-vitro Diagnosis,IVD)未来发展依托的主要检测技术平台之一。微流控芯片技术将大型实验室的各个单元集成在微米,甚至纳米级微结构芯片上,具有体积小、可精确控制流体、试剂消耗少等特点,与IVD整体发展方向高精度、高灵敏度及便携性相契合。本文将从生化、核酸、免疫角度入手,聚焦微流控芯片结构设计及检测原理,总结其在IVD行业中的典型应用、最新进展及未来发展趋势。
High accuracy,high sensitivity in a portable detection device will be the future development direction of in-vitro diagnostic(IVD).Fortunately,microfluidic chip has the characteristics of small volume,precise control of fluid and low reagent consumption owing to its high integration and precise fluid control.Therefore,it will be an ideal platform in the development of IVD.The increasing number of microfluidic-based IVD products were emerging with the fast advance in microfluidics.Importantly,the progressing of the microfluidic chip mainly depends on the innovation of the chip structure and detection principle.This review highlights the progress of microfluidics in IVD techniques from existing microfluidics-based in vitro diagnostic test products to the latest research advances,mainly focus on chip structure and corresponding detection principle in the field of biochemistry diagnosis,molecular diagnosis and immunity diagnosis.
作者
李顺基
肖育劲
陈鹏
刘笔锋
LI Shunji;XIAO Yujin;CHEN Peng;LIU Bifeng(College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074)
出处
《分析科学学报》
CAS
CSCD
北大核心
2020年第5期639-645,共7页
Journal of Analytical Science
基金
国家重点研发计划(No.2016YFF0100801)。
关键词
体外诊断
微流控芯片
生化检测
核酸检测
免疫检测
In-vitro diagnosis
Microfluidic chip
Biochemistry diagnosis
Immunity diagnosis
Molecular diagnosis