摘要
本文中我们采用激光共聚焦光学构型和振镜扫描模式,通过优化光学构型和元件配置,构建了一套细胞扫描成像-荧光互相关光谱联用系统。该系统具有高分辨活细胞成像和原位分析功能,能够用于活细胞内生物分子浓度的测定以及生物分子扩散行为、酶的活性和分子相互作用的研究。进而,我们构建了稳定表达p53-EGFP和MDMX-m Cherry的细胞模型,将构建的系统用于活细胞核内p53-MDMX蛋白相互作用的研究。
In this paper,we reported a confocal scanning imaging-fluorescence correlation spectroscopy system by using confocal optical configuration and optical oscillator scanning mode and optimizing optical configuration and rational configuration of optical components.This system shows two functions:cell imaging with high resolution and in situ fluorescence correlation spectroscopy.Meanwhile,a cell model of stable expression of p53-EGFP and MDMX-mCherry was constructed,and the interaction of the two proteins in the nucleus was investigated by the established system.
作者
董辰博
李福才
董朝青
蔡萍
任吉存
DONG Chenbo;LI Fucai;DONG Chaoqing;CAI Ping;REN Jicun(School of Electronic Information and Electronic Engineering,Shanghai Jiao Tong University,Shanghai 200240;School of Chemistry and Chemical Engineering,Shanghai Jiao Tong University,Shanghai 200240)
出处
《分析科学学报》
CAS
CSCD
北大核心
2020年第5期695-700,共6页
Journal of Analytical Science
基金
国家自然科学基金(No.21874090,21834005)
上海市科委基础研究重点项目(No.18JC1413400)。
关键词
荧光相关光谱
共聚焦扫描成像
活细胞
分子相互作用
Confocal scanning imaging
Fluorescence correlation spectroscopy
Single living cell
Molecular interaction
