基于Fe(Ⅲ)卟啉金属有机框架构建的比色传感器用于H2O2快速检测

Construction of Fe(Ⅲ)Porphyrin Metal Organic Frameworks-Based Colorimetric Sensor for Rapid Detection of H2O2

本文通过一步溶剂热法合成了铁卟啉金属有机框架MIL-141(Fe)纳米酶,基于其高效催化H2O2氧化3,3',5,5'-四甲基联苯胺(TMB)显色,建立了一种简便快速的H2O2比色传感检测方法。系统表征了MIL-141(Fe)纳米酶的形貌、元素组成以及光谱性能,并通过其催化底物的反应动力学研究,得到MIL-141(Fe)纳米酶对过氧化物酶特异性底物TMB和H2O2的米氏常数分别为0.15mmol/L和1.01mmol/L,说明MIL-141(Fe)纳米酶对底物具有高亲和力且响应快速。此外,Fe(Ⅲ)离子与5,10,15,20-四(4-羧基苯基)卟啉(H2TCPP)形成了具有类血红素结构的铁卟啉配体,为催化反应提供了丰富的活性位点。因此,通过催化氧化TMB显色后溶液在波长652nm处吸光度变化情况,实现了对1~150μmol/L浓度范围内H2O2的快速定量分析,检测限为0.60μmol/L。这种基于MIL-141(Fe)纳米酶高催化性能构建的比色传感器为H2O2快速、可视化检测提供了新方法,具有潜在的实际应用价值。

In this work,the Fe(Ⅲ) porphyrin metal organic frameworks(MOFs) nanozyme(MIL-141(Fe)) was synthesized by one-step solvothermal method.Based on its high-efficiency oxidation of 3,3′,5,5′-tetramethylbenzidine(TMB) by catalytic H2O2,a simple and rapid colorimetric method was constructed for the detection of H2O2.The morphology,elemental composition and spectral properties of MIL-141(Fe) nanozyme were characterized systematically.Through steady-state kinetic assays of MIL-141(Fe),the michaelis constant of the MIL-141(Fe) nanozyme to the peroxidase-specific substrates TMB or H2O2 was 0.15 mmol/L or 1.01 mmol/L,respectively.This indicates that MIL-141(Fe) nanozyme has high affinity and rapid response to substrate.In addition,Fe(Ⅲ) ion and 5,10,15,20-tetrakis(4-carboxyphenyl) porphyrin(H2TCPP) form an Fe(Ⅲ) porphyrin ligand with a heme-like structure,providing abundant active sites for nanozyme catalytic reactions.Therefore,with the change of absorbance values at 652 nm after TMB catalytic oxidation,the linearly quantitative analysis of H2O2 with the concentration range from 1 to 150 μmol/L is achieved and the detection of limit is 0.60 μmol/L.This colorimetric sensor based on the high catalytic performance of MIL-141(Fe) nanozyme provides a new method for rapid and visual detection of H2O2,which has potential practical application in the future.