细胞周期蛋白25A在非小细胞肺癌中的表达及与Let-7的关系

Expression of CDC25A in non-small cell lung cancer and its relationship with let-7 gene

目的明确细胞周期蛋白25A(CDC25A)在NSCLC组织中的表达及与临床病理特征的关系,并探讨其与miRNA let-7a1、let-7c表达的相关性。方法收集53例肺组织手术标本,其中包括NSCLC组织44例,收集其癌组织和癌旁正常组织(病理证实),和良性肺疾病组织9例。免疫组化Elivision法检测CDC25A蛋白的表达;用Trizol法提取总RNA,采用荧光定量RT-PCR检测CDC25AmRNA的表达,加尾法荧光定量RT-PCR检测let-7a1和let-7c mRNA的表达。结果CDC25A蛋白表达的阳性率在NSCLC组织明显高于癌旁正常组织和良性肺疾病组织(P<0.05)。NSCLC组织中CDC25A蛋白的表达与年龄、性别、病理类型、肿瘤分化程度、临床分期无关(P>0.05),与吸烟、淋巴结转移相关(P<0.05)。CDC25A mRNA在NSCLC组织中的表达量明显高于癌旁正常组织和良性肺疾病组织(F=6.33,P<0.05),在癌旁正常组织和良性肺疾病组织中表达无明显差异(P>0.05)。Pearson相关分析显示CDC25A与let-7c在NSCLC组织和癌旁正常组织中表达均呈明显负相关(r癌组织=-0.42,r癌旁正常组织=-0.40),与let-7a1之间无明显相关。结论CDC25A在NSCLC组织表达水平明显增高,且与let-7c表达呈明显负相关,推测CDC25A可能是let-7c的下游靶基因。

Objective To investigate the expression of CDC25A in non-small cell lung cancer(NSCLC)tissues and explore its correlation with the clinicpathological features of the patients and the expressions of let-7a1 and let-7c.Methods We collected surgical specimens of pathologically confirmed NSCLC tissues and paired adjacent lung tissues from 44 patients and tissues of benign lung lesions from 9 patients.The expressions of CDC25A protein and mRNA in the tissues were detected by immunohistochemistry and fluorescence quantitative RT-PCR,respectively;the expressions of let-7a1 and let-7c mRNA were detected using tail-adding fluorescence quantitative RT-PCR.Results The positivity rate of CDC25A protein expression was significantly higher in NSCLC tissues than in the adjacent tissues and benign pulmonary lesions(P<0.05).CDC25A protein expression in NSCLC was not correlated with the patients'age,gender,pathological type,degree of tumor differentiation,or clinical stages(P>0.05),and was significantly correlated with smoking and lymph node metastasis(P<0.05).CDC25A mRNA expression was also significantly higher in NSCLC tissues than in the adjacent tissues and benign pulmonary lesions(F=6.33,P<0.05),and was similar between the latter two tissues(P>0.05).Pearson correlation analysis showed that CDC25A expression had a significant negative correlation with let-7c expression in both NSCLC tissues(r=-0.42)and adjacent lung tissues(r=-0.40)but was not correlated with let-7a1 expression.Conclusion The expression level of CDC25A is significantly increased in NSCLC with a negative correlation with Let-7c expression,which identifies CDC25A as a possible downstream target gene of Let-7c.