象山港蓝点马鲛8种同工酶特征分析

Characteristics of eight isozymes in Scomberomorus niphonius from Xiangshan Bay

为探究蓝点马鲛(Scomberomorus niphonius)的生化遗传特征,采用垂直聚丙烯酰胺凝胶电泳技术分析了6尾象山港蓝点马鲛个体(3雄,3雌)的9种组织(心脏、鳃、肠、肌肉、胃、肝、眼、肾和性腺)中乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、葡萄糖脱氢酶(GDH)、葡萄糖6磷酸脱氢酶(G6PDH)、超氧化物歧化酶(SOD)、酯酶(EST)、山梨醇脱氢酶(SDH)和苹果酸酶(MEP)等8种同工酶的表达模式。结果表明,同工酶呈现出组织特异性,LDH、MDH和G6PDH在9种组织中均有表达,GDH在肝脏和眼中弱表达;在各种组织中,肝脏中同工酶表达最广泛,8种同工酶均有表达,其中EST的酶条带比较丰富,GDH和G6PDH在肝脏中活性较高。各种同工酶表达谱在雌雄个体组织间无差别。研究结果可为象山港蓝点马鲛的种质鉴定提供一定的理论依据。

In order to explore the biochemical and genetic characteristics of Scomberomorus niphonius,Gradient Polyacrylamide Gel Electrophoresis(GPGE)was used to analyze the expression patterns of eight isozymes(LDH,MDH,GDH,G6 PDH,SOD,EST,SDH and MEP)in nine tissues(cardiac,gill,intestine,muscle,stomach,liver,eyes,kidney and gonad)of six individuals(3 females,3 males)from Xiangshan Bay.The results showed that all eight isozymes were tissue-specific expression in nine tissues.LDH,MDH and G6 PDH expressed in all nine tissues,while GDH only expressed little in the liver and eyes.Isoenzyme expressions were most abundant in liver among nine tissues.Eight isozymes were detected in the liver,in which the enzyme bands of EST were most abundant.GDH and G6 PDH were more active in the liver than in other tissues.Isozyme expression patterns had no difference between male and female.The results provide a theoretical basis for the germplasm identification of Scomberomorus niphonius.