ZFP36和Rasd1的mRNA鉴别生前、死后挫伤的意义

Forensic application of ZFP36 and Rasd1 mRNAs in identifying antemortem and postmortem skin contusion

目的探索ZFP36和Rasd1在鉴别生前与死后皮肤挫伤上的法医学应用价值。方法使用实时定量聚合酶链式反应(RT-qPCR)的方法,对小鼠和人类皮肤组织中ZFP36和Rasd1的表达情况进行检测。结果在动物实验中,与空白对照和死后挫伤相比,生前挫伤处皮肤ZFP36和Rasd1的mRNA水平明显升高,且二者升高水平在死后72h仍可检出。为进一步验证两者的法医实际应用价值,本实验使用15例法医尸体解剖材料进行了验证。和自身未受损处皮肤相比,死后时间较短(PMI<48h)的10例尸体检材中,有9例ZFP36和Rasd1的mRNA水平在损伤处明显升高;而死亡时间较长(PMI≥60h)的5例尸体材料中,损伤处ZFP36和Rasd1的mRNA水平没有明显变化。结论ZFP36和Rasd1有望作为生前挫伤生活反应的指标应用于法医实践。

Objective To explore the forensic application value of ZFP36 and Rasd1 in identifying antemortem and postmortem skin contusion.Methods Through RT-qPCR,this study investigated mRNA levels of ZFP36 and Rasd1 in mouse and human skin wounds.Results In animal experiments,the mRNA levels of skin ZFP36 and Rasd1 increased significantly in antemortem contused mouse skins,compared with blank controls and postmortem contusions.The increased mRNA expressions can still be observed 72 hours after death for ZFP36 and Rasd1 in contused mouse skin,respectively.To further validate the value of both forensic applications,15 postmortem human skin tissues were also examined.In human wounded samples,increased ATF3 and Rasd1 mRNA levels were detected in 9 of 10 cases with short postmortem intervals(PMI<48 h),and there was no significant change in 5 cases with long postmortern intervals(PMI≥60 h).Conclusion These findings suggest that the detection of mRNA levels of ZFP36 and Rasd1 might be used in forensic practice as a potential marker for vital reaction to contusion.