超声造影参数与分化型甲状腺癌肿瘤组织中增殖基因表达及淋巴结转移的关联性分析

Association Analysis of Ultrasound Contrast Parameters with Proliferation Gene Expression and Lymph Node Metastasis of Differentiated Thyroid Cancer

本研究中选取分化型甲状腺癌患者82例为恶性组,82例良性甲状腺结节患者为良性组,均实施超声造影,并检测分化型甲状腺癌患者肿瘤组织中增殖基因表达及淋巴结转移情况。结果发现,恶性组患者的PI、MTT、TTP均值低于良性组,肿瘤组织标本中Twist1、TPX2 mRNA表达量均值高于良性组,PP4R1、PDCD4 mRNA表达量均值低于良性组。PI、MTT、TTP水平与肿瘤组织标本中Twist1、TPX2 mRNA表达量呈负相关,与PP4R1、PDCD4 mRNA表达量呈正相关(P<0.05)。PI、MTT、TTP联合预测分化型甲状腺癌患者淋巴结转移的AUC值高于单一指标预测。上述结果提示超声造影参数PI、MTT、TTP异常程度与肿瘤增殖基因异常表达及淋巴结转移直接相关,超声造影检查可量化反映甲状腺癌肿瘤细胞增殖及侵袭活性。

In this paper,82 patients with differentiated thyroid cancer were selected as the malignant group,and 82 patients with benign thyroid nodules were selected as the benign group.All were performed ultrasound contrast,as well as the detection of proliferation gene expression in tumor tissue and lymph node metastasis of differentiated thyroid cancer.It was found that the levels of PI,MTT,and TTP in the malignant group were lower than those in the benign group,while the expression levels of Twist1 and TPX2 mRNA in tumor specimens in the malignant group were higher than those in the benign group.In addition,the expression levels of PP4R1 and PDCD4 mRNA were lower than those in the benign group.The ultrasound contrast parameters were negatively correlated with the expression of Twist1 and TPX2 mRNA in tumor tissue samples,and positively correlated with the expression of PP4R1 and PDCD4 mRNA(P<0.05).The AUC value of combination of PI,MTT and TTP to predict the lymph node metastasis of differentiated thyroid cancer was higher than that predicted by a single index.It is suggested that the abnormalities of ultrasound contrast parameters are directly related to the changed expression of proliferating genes and lymph node metastasis.By ultrasonography,we can quantify the proliferation and invasion activity of thyroid cancer tumor cells.