黄芪注射液对不同子痫前期样模型小鼠CRP、IL-6、TNF-α表达的影响

Effect of Huangqi injection on the expression of CRP,IL-6 and TNF-αin different preeclampsia-like model mice

目的研究黄芪注射液对不同子痫前期(PE)样模型小鼠C反应蛋白(CRP)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)的影响。方法给予C57BL/6J小鼠皮下注射亚硝基左旋精氨酸甲酯(L-NAME)或腹腔内注射脂多糖(LPS)建立PE样小鼠模型,同期注射生理盐水作为正常对照。在小鼠妊娠第8天开始给予黄芪注射液(L-NAME+黄芪注射液组、LPS+黄芪注射液组、正常+黄芪注射液组)或生理盐水灌胃(L-NAME+生理盐水组、LPS+生理盐水组、正常+生理盐水组),1次/d。在小鼠妊娠第18天,收集各组孕鼠的血液肝脏及胎盘组织,通过酶联免疫吸附(ELISA)、Western blotting比较CRP、IL-6、TNF-α在各组孕鼠肝脏、胎盘组织及血清中的表达水平。结果各组小鼠血清、肝脏组织以及胎盘组织中:L-NAME+生理盐水组、LPS+生理盐水组较正常+生理盐水组,CRP、IL-6、TNF-α表达水平均明显上升,差异有统计学意义(P<0.05);L-NAME+黄芪注射液组较L-NAME+生理盐水组、LPS+黄芪注射液组较LPS+生理盐水组,CRP、IL-6、TNF-α表达水平均明显下降,差异有统计学意义(P<0.05)。L-NAME+生理盐水组、L-NAME+黄芪注射液组、LPS+生理盐水组、LPS+黄芪注射液组CRP与IL-6、TNF-α均呈显著正相关(P<0.01)。结论黄芪注射液可降低不同PE样模型小鼠CRP、IL-6、TNF-α的表达水平,有助于对PE炎症反应水平进行控制,从而延缓病情的进展。

Objective To investigate the effects of astragalus injection on C reactive protein(CRP),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)in different preeclampsia(PE)-like model mice.Methods C57BL/6J mice were given subcutaneous injection of nitrite levarginine methyl ester(L-NAME)or intraperitoneal injection of lipopolysaccharide(LPS)to establish a preeclampsia-like mice model with simultaneous injection of normal saline as a normal control;astragalus membranaceus injection(L-NAME astragalus injection group,LPS astragalus injection group,normal astragalus injection group)or normal astragalus membranaceus injection group(normal saline group,LPS saline group,normal saline group)was administered once a day on the 8th day of pregnancy.On the 18th day of pregnancy,the liver,placental tissue and blood of pregnant mice were collected,and the expression level of CRP,IL-6 and TNF-α protein in liver,placental tissue and serum was compared by ELISA,Western blot.Results In the mouse serum,liver tissue and placenta tissue of each group:Compared with the normal+normal saline group,the expression levels of CRP,IL-6 and TNF-α in the L-NAME+normal saline group,LPS+normal saline group increased significantly(P<0.05);Compared with L-NAME+Astragalus injection group,L-NAME+saline group and LPS+Astragalus injection group compared with LPS+saline group,the expression levels of CRP,IL-6 and TNF-α were significantly decreased(P<0.05).CRP was significantly positively correlated with IL-6 and TNF-α in the L-NAME+normal saline group,L-NAME+Astragalus injection group,LPS+normal saline group,LPS+Astragalus injection group(P<0.01).Conclusion Astragalus injection can reduce the expression level of CRP,IL-6 and TNF-αprotein in preeclampsia-like model mice,which helps to control the level of inflammation in mice with preeclampsia,thereby delaying the progression of the disease.