不同浓度增效剂对金龟子绿僵菌、黄绿绿僵菌和球孢白僵菌产孢的影响

Effects of Different Concentrations of Synergists on Sporulation of Metarhizium anisopliae,Metarhizium flavoviride and Beauveria bassiana

生防菌作为一种重要的生物杀虫剂,被开发成多种制剂应用于农林害虫的防治;微生物制剂的生产成本高、耗时长,极大影响其生产效率。本试验通过液固两相发酵法筛选出提高金龟子绿僵菌Metarhizium anisopliae、黄绿绿僵菌Metarhizium flavoviride和球孢白僵菌Beauveria bassiana产孢量的最优增效剂浓度。结果表明0.1%浓度增效剂对金龟子绿僵菌的促产孢作用达最大化,浓度达4.48×109孢子/g;黄绿绿僵菌的最适增效剂浓度为0.05%,浓度达2.75×109孢子/g;当增效剂浓度小于0.1%时利于黄绿绿僵菌的产孢,增效剂高于0.1%时抑制产孢;而球孢白僵菌的产孢量与增效剂浓度正相关,最大浓度达7.41×109孢子/g。在真菌液体发酵过程中添加产孢增效剂,有效提高了真菌产孢量,缩短了真菌培养周期,为提高真菌制剂生产效率、节约成本提供理论支持。

Certain fungus has shown effective control as biological pesticide for various agricultural and forest pests.However,production of microbial preparations is comparatively expensive and time-consuming,which greatly affects the production efficiency.In this study,the optimal synergist concentration to increase the spore production of Metarhizium anisopliae,Metarhizium flavoviride,and Beauveria bassiana by liquid-solid two-phase fermentation was screened.The results showed that 0.1%synergist had the highest spore-promoting effect on M.anisopliae,with a spore concentration of 4.48×109 per gram.Optimal synergist concentration for M.anisopliae was 0.05%,which produced spore concentration of 2.75×109 per gram.Synergist concentration of<0.1%was beneficial for spore production of M.anisopliae,whereas concentration of>0.1%inhibited the sporulation.In contrast,the spore production of B.bassiana was positively correlated with the concentration of synergist,reaching the maximum spore concentration of 7.41×109 per gram.Adding sporulation synergists in the fungal liquid fermentation process has effectively enhanced the fungal spore production,shortened the fungal culture cycle,and thus improved the production efficiency and reduced production cost of fungal preparations.