摘要
分别将深绿木霉(Trichoderma atroviride)菌丝、分生孢子悬浮液接种和喷洒到携带Lentinula edodes mycovirus HKB(LeV-HKB)的3个香菇(Lentinula edodes)菌株,及其相应等基因脱毒菌株的菌落上,比较了25℃和37℃(高温胁迫)条件下处理的香菇带毒和脱毒菌株对深绿木霉菌丝和分生孢子的抗性,以及对深绿木霉产孢能力的影响。结果表明:25℃的处理,香菇菌株是否带毒对深绿木霉的菌丝和分生孢子的抗性及对深绿木霉菌丝产孢能力的影响差异不显著;经37℃处理48 h后,香菇带毒菌株SY1和S606对深绿木霉菌丝和分生孢子的抗性,以及对深绿木霉菌丝产孢能力的影响显著弱于其脱毒菌株SY1-R8和S606-M8,而带毒菌株L135在这3个性状上显著强于其脱毒菌株L135-M8。本研究说明,LeV-HKB影响受高温胁迫后的香菇菌丝对深绿木霉的抗性,但因寄主不同影响效果不同。
Using three Lentinula edodes strains carrying L.edodes mycovirus HKB(LeV-HKB),SY1,S606 and L135,and their corresponding isogenic virus-free strains,SY1-R8,S606-R8 and L135-R8,as experimental subjects,effects of LeV-HKB on resistance of heat stress challenged L.edodes against Trichoderma atroviride were studied.Colonies of L.edodes were incubated at 25℃(control)or 37℃(heat stress)for 2 d prior to manual inoculation with either mycelial blocks or conidial suspension of T.atroviride,and then these colonies were further incubated at 25℃for 10 d in the case of L.edodes inoculated with mycelial blocks and 15 d in the case of L.edodes inoculated with conidial suspension.Resistance to mycelial growth of T.atroviride was analyzed 5 d after inoculation with mycelial blocks of T.atroviride by measuring the browning rate and influence index of each L.edodes culture.Resistance to sporulation of T.atroviride was analyzed by taking photos of all the cultures 10 d after inoculation with mycelial blocks.Resistance to conidia production of T.atroviride was analyzed by taking photos of all the cultures 7 d and 15 d after inoculation with conidial suspension of T.atroviride,respectively.The results showed that all the strains carrying LeV-HKB had no significant difference with their corresponding isogenic virus-free strains on resistance to T.atroviride when cultivated at 25℃for the first 2 d.Upon heat stress challenge,SY1 and S606 were more susceptible to T.atroviride infection than their corresponding isogenic virus-free strains SY1-R8 and S606-R8,respectively.In contrast,L135 showed better resistance against T.atroviride than L135-R8.These results suggest that LeV-HKB plays a role in the resistance of heat stress challenged L.edodes against T.atroviride with varied effects in different strains.
作者
郭孟配
叶征明
申桂煜
边银丙
徐章逸
GUO Mengpei;YE Zhengming;SHEN Guiyu;BIAN Yinbing;XU Zhangyi(Institute of Applied Mycology,Huahong Agricultural University,Wuhan Hubei 430070 China)
出处
《食用菌学报》
CSCD
北大核心
2020年第4期143-148,共6页
Acta Edulis Fungi
基金
国家重点研发计划子课题(2019YFD1001905-35)
国家现代农业产业技术体系岗位科学家专项(CARS-20)。
