健康供者异基因外周血造血干细胞移植采集因素分析

Collection factors associated with allogeneic peripheral blood hematopoietic stem cell yield in healthy donors

目的干细胞的足量采集是异基因外周血造血干细胞(allo-PBSCs)成功移植的重要因素。本研究分析比较了健康供者异基因外周血造血干细胞动员是否良好的采集相关因素。方法回顾性分析了71例于新桥医院在2017—2019年进行的异基因外周血造血干细胞采集的临床数据。分析以CD34+细胞作为研究指标,包括年龄、动员前和采集前血常规结果、干细胞产品相关数据等。CD34+细胞数量>4.0×10^6/Kg被认为是异基因外周血造血干细胞成功移植的最小剂量,也是本研究的靶剂量。本文以此为界,分为两组健康供者。结果 CD34+细胞相关因素是年龄、WBC、Plt,动员良好的健康供者采集产品中所获得的单个核细胞(mononuclear cells,MNC)数量和CD34+数量较高。结论本研究认为年轻的健康供者、采集前有较高的WBC和Plt数量,能获得较高的CD34+细胞数量,对成功进行异基因外周血造血干细胞移植有重要意义。

Objective Sufficient collection of stem cells was an important factor for the successful transplantation of allogeneic peripheral blood stem cells(allo-PBSCs). This study analyzed and compared factors associated with the good mobilization of allo-PBSCs in healthy donors. Methods The clinical data of 71 patients who underwent allo-PBSCs collection in Xinqiao Hospital from 2017 to 2019 were retrospectively analyzed. CD34+ cells were used as the study indicators, and its relationship with certain donor characteristics, including age, routine blood results before mobilization and harvesting, data regarding stem cells products, etc. was analyzed. The yield of CD34+ cells >4.0×10^6/Kg was considered as the minimum dose for successful transplantation of allo-PBSCs, which was also the target dose of this study. In this study, we used the target dose as the boundary to divide healthy donors into two groups. Results The donor′s age, WBC count, platelet count were correlated with the yield of CD34+ cell. A higher yield of MNC and CD34+ cells was observed in well-mobilized healthy donors.Conclusion This study reveals that young healthy donors with higher counts of WBC and Plt before mobilization usually predict a higher CD34+ cell yield, which was of great significance in successful allo-PBSC transplantation.