西瓜枯萎病田间调查及其病原菌鉴定

Field Investigation and Pathogen Identification of Watermelon Fusarium Wilt

对福建省4个地区种植西瓜实生苗田块的枯萎病发病率进行调查,结果表明:西瓜枯萎病的田间平均发病率在18.5%~38.2%。从不同地点采集的发病植株上共分离、纯化和保存了31株菌株,结合菌株的形态学特征、镰刀菌属特异引物Fu3/Fu4和尖孢镰刀菌特异性引物FOF1/FOR1检测以及致病性测定,确定了尖孢镰刀菌Fusarium oxysporum为福建省西瓜枯萎病的主要病原菌,其中尖孢镰刀菌占总分离菌株数量的93.54%。

The incidence of Fusarium wilt in the fields of planting the watermelon seedlings at the 4 regions of Fujian Province was investigated・The results showed that the average incidences of Fusarium wilt was from 18.5%to 38.2%in the different fields.A total of 31 strains were isolated,purified and preserved from the diseased plants collected from the different sites.Fusarium oxysporum was identified as the main pathogen of Fusarium wilt of watermelon in Fujian Province based on the morphological characteristics,PCR amplification of the specific primers Fu3/Fu4 for Fusarium gene and FOF1/FOR1 for Fusarium oxysporum,and the pathogenicity test.And Fusarium oxysporum accounted for 93.54%of the total number of isolated strains.