miRNA-196a对宫颈癌细胞裸鼠移植瘤生长的影响调节作用及相关机制研究

Effects of miRNA-196a on the growth of cervical cancer xenografts in nude mice and related mechanisms

目的探讨与研究miRNA-196a对宫颈癌细胞裸鼠移植瘤生长的影响调节作用及相关机制。方法36只小鼠平分为3组:对照组、实验1组、实验2组,每组各12只。实验1组、实验2组分别经腹腔注射0.2 m L感染Has慢病毒空载体、Has-miRNA-196a慢病毒载体的Hela细胞,对照组注射等体积的Hela细胞,连续注射3 d,在注射后4周测定移植瘤生长情况并检测细胞凋亡、自噬与炎症因子表达情况。结果实验2组的移植瘤重量(4.01±0.22)g低于实验1组(6.23±0.22)g与对照组(6.26±0.53)g,抑瘤率(40.86±11.31)%、细胞凋亡指数(30.67±2.14)%、Beclin-1(16.43±2.91)、LC3(12.22±1.13)相对表达水平高于实验1组[(40.86±11.31)%、(3.20±0.11)%、1.11±0.13、1.24±0.21]与对照组[(0.00±0.00)%、(3.13±0.24)%、1.13±0.03,1.23±0.321],差异有统计学意义(P<0.05),实验1组与对照组比较,差异无统计学意义(P>0.05)。实验2组的血清肿瘤坏死因子α(TNF-α)(7.21±0.52)pg/m L与白细胞介素6(IL-6)(8.08±0.54)pg/m L含量显著低于实验1组[(27.03±1.10)pg/m L、(35.81±1.66)pg/m L]与对照组[(27.33±1.49)pg/m L、(34.20±1.11)pg/m L],差异有统计学意义(P<0.05),实验1组与对照组比较,差异无统计学意义(P>0.05)。结论MiRNA-196a在宫颈癌细胞裸鼠移植瘤中的过表达能促进移植瘤的凋亡与自噬,抑制肿瘤细胞炎症因子的释放,提高抑瘤率,从而降低移植瘤重量。

Objective To investigate and study the effect of miRNA-196 a on the growth of cervical cancer xenografts in nude mice and related mechanisms.Methods Thirty-six mice were divided into three groups:control group,experimental group 1 and experimental group 2 with 12 cases in each group.Experimental group 1 and experimental group 2 were injected intraperitoneally with 0.2 m L Hela cells infected with Has lentiviral empty vector and Has-miRNA-196 a lentiviral vector,and the control group were injected with equal volume of Hela cells for 3 consecutive days,4 weeks after injection,Measured the growth of transplanted tumors,detected the expression of apoptosis,autophagy and inflammatory factors.Results The weight of the transplanted tumor in the experimental group(4.01±0.22)g,were lower than that in the experimental group 1(6.23±0.22)g and the control group(6.26±0.53)g,and the tumor suppression rate(40.86±11.31)%,apoptosis index(30.67±2.14)%,relative expression level of Beclin-1(16.43±2.91)and LC3(12.22±1.13)were higher than those in the experimental group 1[(40.86±11.31)%,(3.20±0.11)%,1.11±0.13,1.24±0.21]and the control group[(0.00±0.00)%,(3.13±0.24)%,1.13±0.03,1.23±0.321],the difference was statistically significant(P<0.05).There were no significant difference compared between the experimental group 1 and the control group(P>0.05).The levels of serum TNF-α(7.21±0.52)pg/m L and IL-6(8.08±0.54)pg/m L in the experimental group 2 were significantly lower than those in the experimental group 1[(27.03±1.10)pg/m L,(35.81±1.66)pg/m L]and the control group[(27.33±1.49)pg/m L,(34.20±1.11)pg/m L],the difference was statistically significant(P<0.05).There were no statistically significant difference compared between the experimental group 1 and the control group(P>0.05).Conclusion Over-expression of miRNA-196 a in cervical cancer xenograft tumors can promote the apoptosis and autophagy of transplanted tumors,inhibit the release of inflammatory factors of tumor cells,increase the tumor suppression rate,and thus reduce the weight of transplanted tumors.