环磷酰胺对百草枯中毒家兔肺过氧化损伤的干预作用

Intervention Effect of Cyclophosphamide on Lung Peroxidation Injury Induced by Paraquat in Rabbits

目的:探讨环磷酰胺对百草枯中毒家兔肺过氧化损伤的干预作用。方法:将40例健康家兔随机分为对照组、模型组、甲强龙组、环磷酰胺+甲强龙组,每组10例。模型组、甲强龙组、环磷酰胺+甲强龙组均给予百草枯灌胃染毒,甲强龙组染毒后给予甲强龙治疗,环磷酰胺+甲强龙组给予环磷酰胺和甲强龙,对照组和模型组腹腔注入生理盐水。采集各组动物治疗1d、3d、5d、7d时耳缘静脉血,检测血清TGF-β1、MDA及SOD水平。结果:治疗1d后,模型组、甲强龙组、环磷酰胺+甲强龙组血清TGF-β1、MDA水平均高于对照组,血清SOD水平则低于对照组(P<0.05)。治疗3d、5d、7d后,甲强龙组、环磷酰胺+甲强龙组血清TGF-β1、MDA水平均低于模型组,血清SOD水平则高于模型组(P<0.05)。环磷酰胺+甲强龙组在治疗3d、5d、7d后血清TGF-β1、MDA水平均低于甲强龙组,血清SOD水平则高于甲强龙组(P<0.05)。结论:环磷酰胺能有效降低血清TGF-β1、MDA的表达和提高SOD活性,从而改善百草枯中毒引起的过氧化损伤。

Objective:To investigate the effect of cyclophosphamide on lung peroxidation injury of rabbits with paraquat poisoning.Methods:40 healthy rabbits were randomly divided into control group,model group,methylpolygonon group and cyclophosphamide+methylpolygonon group,with 10 cases in each group.Paraquat was given to the model group,methylpolygonon group and cyclophosphamide+methylpolygonon group.The methylprednisolone group was treated with methylprednisolone,the cyclophosphamide+methylprednisolone group was treated with cyclophosphamide and methylprednisolone,and the control group and model group were given normal saline.Results:After 1 day of treatment,the levels of TGF-β1 and MDA in model group,methylprednisolone group and cyclophosphamide+methylprednisolone group were higher than those of control group,while serum SOD level was lower than that of control group(P<0.05).After 3 days,5 days and 7 days of treatment,the levels of serum TGF-β1 and MDA in methylprednisolone group and cyclophosphamide+methylprednisolone group were lower than those of model group,while serum SOD level was higher than that of model group(P<0.05).The levels of serum TGF-β1 and MDA in the cyclophosphamide+methylprednisolone group were lower than those in the methylprednisolone group after 3 days,5 days and 7 days of treatment,while the serum SOD level was higher than that of the methylprednisolone group(P<0.05).Conclusion:Cyclophosphamide can effectively reduce the expression of serum TGF-β1 and MDA and increase the activity of SOD,so as to improve the peroxidation damage caused by paraquat.