浓缩生长因子对缺血再灌注诱导的小鼠骨样细胞凋亡的作用研究

Concentrated Growth Factor Promotes Angiogenic Potential of Endothelial Cells in Vitro

目的:研究浓缩生长因子(CGF)对小鼠骨样细胞(MLO-Y细胞)的凋亡的作用。方法:应用健康志愿者静脉血制成的CGF制备浓缩生长因子提取液(CGFe)。复制体外缺血再灌注模型,分为空白对照组和不同浓度处理的实验组(包括1%CGFe,5%CGFe,10%CGFe)。通过试剂盒检测caspase-3活性,实时荧光定量聚合酶链反应(qRT-PCR)检测各组Bax,Bcl-2及p53的mRNA表达量评价MLO-Y4细胞凋亡的程度。结果:CGF能够明显抑制缺血再灌注导致的MLO-Y4细胞caspase-3活性的升高,伴随Bax,Bcl-2,p53的mRNA水平的降低。结论:CGF能够明显改善缺血再灌注诱导的MLO-Y4细胞的凋亡。

Objective:To study the effect of concentrated growth factor(CGF)on the apoptosis of MLO-Y4 osteocyte-like cells.Methods:CGF was prepared from the venous blood of healthy volunteers.The model of ischemia-reperfusion in vitro was duplicated and divided into control and experimental groups with different concentrations(including 1%CGFe,5%CGFe,10%CGFe).The expression levels of Bax,Bcl-2 and p53 mRNA were detected by real-time fluorescence quantitative polymerase chain reaction(qRT PCR)to evaluate the degree of MLO-Y4 osteocyte-like cells apoptosis.Results:CGF could significantly inhibit the increase of caspase-3 activity of MLO-Y4 osteocyte-like cells induced by ischemia-reperfusion,with the decrease of Bax,Bcl-2 and p53 mRNA levels.Conclusion:CGF can improve the apoptosis of human umbilical vein endothelial cells induced by ischemia-reperfusion.