摘要
目的:研究青刺尖的石油醚部位、乙酸乙酯部位、30%乙醇部位和60%乙醇部位对4种细菌的体外抑菌作用。方法:采用二倍稀释法初步检测青刺尖的不同萃取物对金黄色葡萄球菌、表皮葡萄球菌、大肠埃希菌和肺炎克雷伯菌的抑菌浓度范围。结果:青刺尖石油醚部位在生药浓度分别为0.0391~2.5000 g﹒mL^-1、0.1563~2.5000 g﹒mL^-1和0.1563~2.5000g﹒mL^-1的范围内对金黄色葡萄球菌、表皮葡萄球菌和肺炎克雷伯菌的抑制作用存在剂量依赖性,青刺尖乙酸乙酯部位在生药浓度分别为0.1563~2.5000 g﹒mL^-1、0.3125~2.5000 g﹒mL^-1、1.2500~2.5000 g﹒mL^-1和0.3125~2.5000 g﹒mL^-1的范围内对金黄色葡萄球菌、表皮葡萄球菌、大肠埃希菌和肺炎克雷伯菌的抑制作用存在剂量依赖性。青刺尖30%乙醇部位和60%乙醇部位无明显抑菌作用。结论:青刺尖石油醚部位和乙酸乙酯部位有一定的体外抑菌作用,且对革兰氏阳性菌的抑制作用强于对革兰氏阴性菌的抑制作用。
Objective:To study the bacteriostatic effect of the petroleum ether extract,ethyl acetate extract,30%ethanol extract and 60%ethanol extract of Qingcijian on four kinds of bacteria in vitro.Method:The inhibition range of different extracts of Qingcijian on staphylococcus aureus,staphylococcus epidermidis,escherichia coli,and klebsiella pneumoniae were detected by double-fold dilution method.Result:The petroleum ether extract had a dose-dependent bacteriostatic effect on staphylococcus aureus,staphylococcus epidermidis,and klebsiella pneumoniaein the range of crude drug concentrations of 0.0391-2.5000g﹒mL^-1,0.1563-2.5000 g﹒mL^-1 and 0.1563-2.5000 g﹒mL^-1.The ethyl acetate extract had a dose-dependent bacteriostatic effect on staphylococcus aureus,staphylococcus epidermidis,escherichia coli,and klebsiella pneumoniae in the range of crude drug concentrations of 0.1563~2.5000 g﹒mL^-1,0.3125~2.5000 g﹒mL^-1,1.2500~2.5000 g﹒mL^-1and 0.3125~2.5000 g﹒mL^-1.There was no obvious bacteriostatic effect on the 30%ethanol extract and 60%ethanol extract.Conclusion:The petroleum ether extract and ethyl acetate extract of Qingcijian have a certain bacteriostatic effect in vitro,and the bacteriostatic effect on gram-positive bacteria is stronger than that on gram-negative bacteria.
作者
杨天龙
郑振兴
张传洋
王迪
杨欢
邓放
YANG Tian-long;ZHENG Zhen-xing;ZHANG Chuanyang;WANG Di;YANG Huan;DENG Fang(School o f Pharmacy,Chengdu University of Traditional Chinese Medicine,Key Laboratory of Standardization for Chinese Herbal Medicine,Ministry of Education,National Key Laboratory Breeding Base of Systematic Research,Development and Utilization of Chinese Medicine Resources,Chengdu 611137,Sichuan)
出处
《中药与临床》
2020年第4期21-23,共3页
Pharmacy and Clinics of Chinese Materia Medica
基金
四川省科技厅省青年科技创新研究团队专项计划(2016TD0006)。
关键词
青刺尖
抑菌作用
金黄色葡萄球菌
大肠埃希菌
表皮葡萄球菌
肺炎克雷伯菌
Qingcijian
bacteriostatic effect
staphylococcus aureus
staphylococcus epidermidis
escherichia coli
klebsiella pneumoniae