煤矿区源Penicillium sp.XR046真菌次生代谢产物化学成分研究

Study on the Chemical Constituents of Secondary Metabolites from Penicillium sp.XR046 Collected in Coal Area

对贵州省兴仁市煤矿区土壤源Penicillium sp.XR046真菌代谢物中化学成分进行研究,为筛选活性先导化合物提供化学分子来源。采用大米固体培养对真菌Penicillium sp.XR046进行发酵培养,经乙酸乙酯提取获得总代谢产物。通过联合运用硅胶柱层析、Sephadex LH-20、制备高效液相等现代分离技术对其代谢物中的化学成分进行分离,运用NMR和MS现代波谱技术对单体化合物的结构进行鉴定。从Penicillium sp.XR046真菌代谢物中分离得到20个化合物。其中12个化合物为首次从青霉属真菌代谢物中分离。丰富了Penicillium sp.次生代谢物化合物库,为相关药理活性筛选奠定基础。

Chemical constituents from the metabolites of the Penicillium sp.XR046 which was collected in Xingren coal area of Guizhou province to provide source for screening active compounds was investigated.The fungus Penicillium sp.XR046 was cultured by solid-state fermentation and the total metabolites were obtained by extraction with ethyl acetate.All compounds were isolated by comprehensive chromatography such as silica gel CC,Sephadex LH-20 CC,and semi-preparative HPLC,and were identified by NMR and MS spectra.Twenty known compounds were isolated and identified from metabolites of Penicillium sp.XR046.Twelve compounds were firstly isolated from the metabolites of the Penicillium sp.genus.The study enriched the chemical constitutes data of Penicillium sp.and provided foundation for the screening of pharmacological activities.