超声辅助乙醇提取荔枝核总皂苷工艺优化及其抗炎作用研究

Ultrasonic Assisted Ethanol Extraction Method and Anti-inflammatory Activity of Total Saponins from Litchi Seeds

优选超声辅助乙醇提取荔枝核总皂苷的工艺条件,并研究其抗炎活性。在单因素的基础上,以乙醇浓度、提取时间、料液比为影响因素,通过正交试验优化提取工艺。进而以LPS诱导RAW 264.7细胞为炎症模型,考察其抗炎活性。最优提取工艺为70%乙醇为溶剂,料液比为1∶20,超声辅助提取为30 min,总皂苷的含量为4.61 mg/g;抗炎活性研究结果表明荔枝核总皂苷在2~80μg/mL未表现出细胞毒性,但对LPS诱导RAW264.7细胞导致的NO和IL-6的释放均有很好的抑制效果。超声辅助乙醇提取荔枝核总皂苷工艺稳定可靠,而且具有较低的细胞毒性和较好的抗炎活性。

To optimize the conditions of total saponins extracted from Litchi seed by ultrasound-assisted ethanol extraction and then evaluated its anti-inflammatory activity.On the basis of single factor,the orthogonal experiment was used to optimize the extraction of total saponins from Litchi seed and the concentration of ethanol,extraction time and solid-liquid ratio were selected as influence factors.The anti-inflammatory activity of total saponins was evaluated via RAW 264.7 cells induced by LPS.The optimal conditions were concluded as follows:ethanol concentration was 70%,ratio of liquid to solid was 20∶1,and extraction time was 30 min.The mean concentration of total saponins extracted by optimal conditions was 4.61 mg/g.The total saponins from Litchi seed were shown no cytotoxic activity in 2~80μg/mL while showed significant NO and IL-6 inhibition activity via RAW 264.7 cells induced by LPS.Ultrasound-assisted ethanol extraction of total saponins from Litchi seed was stable and reliable,and has low cytotoxicity and good anti-inflammatory activity.