摘要
利用改良索氏提取器,研究了柠檬酸诱导人参须根皂苷转化为人参皂苷Rg3的方法,并以人参皂苷20(R/S)-Rg3总得率为考察指标,通过单因素实验进行了工艺优化,同时探索了原人参二醇组皂苷到人参皂苷Rg3的生成路径。优化柠檬酸诱导人参须根皂苷转化为人参皂苷Rg3的制备工艺并探索Rg3生成路径。实验结果表明,酸浓度0.16 mol/L、固液比1∶15(g/mL)、蒸馏瓶加热温度110℃、提取桶内加热温度65℃、60%甲醇溶液中提取3 h,20(R)-Rg3和20(S)-Rg3总得率为2.51%。柠檬酸诱导下,原人参二醇组皂苷不经过人参皂苷Rd,而是直接水解C-20位双糖生成Rg3。在人参皂苷提取过程中通过一步法直接完成人参粉中人参皂苷的提取、人参皂苷的结构修饰、人参粉与人参提取液的分离,可以显著提高稀有人参皂苷Rg3的制备效率,方法简单,可以为大量生产人参皂苷Rg3提供理论和技术支持。
Using an improved soxhlet extractor,the method of citric acid inducing the conversion of Fibrous Root Ginsenoside into ginsenoside Rg3 was studied,and the total yield of ginsenoside 20(R/S)-Rg3 was used as an indicator to optimize the process through single factor experiments.At the same time,the production path of protopanaxadiol saponins to ginsenoside Rg3 was explored.Optimize the preparation process of citric acid-induced conversion of Fibrous Root Ginsenoside into ginsenoside Rg3 and explore the production pathway of Rg3.The experimental results showed that the acid concentration was 0.16 mol/L,the solid-toliquid ratio was 1∶15(g/mL),the heating temperature of the distillation flask was 110℃,the heating temperature in the extraction barrel was 65℃,and the extraction was carried out in 60%methanol solution for 3 h.The total yield of 20(R)-Rg3 and 20(S)-Rg3 is 2.51%.Under the induction of citric acid,the protopanaxadiol saponins did not undergo ginsenoside Rd,but directly hydrolyzed the C-20 disaccharide to generate Rg3.In the process of ginsenoside extraction,the extraction of ginsenosides in ginseng powder,the structural modification of ginsenosides,and the separation of ginseng powder and ginseng extract liquid can be completed directly by one-step method in the extraction process of ginsenosides.The preparation efficiency of rare ginsenoside Rg3 can be significantly improved.The method is simple and can provide theoretical and technical support for mass production of ginsenoside Rg3.
作者
张仔豪
叶安琪
何治平
唐良峥
李杰
董明月
成乐琴
ZHANG Zi-hao;YE An-qi;HE Zhiping;TANG Liang-zheng;LI Jie;DONG Ming-yue;CHENG Le-qin(Department of Pharmacy and Applied Chemistry,Jilin Institute of Chemical Technology,Jilin 132022,China)
出处
《化学试剂》
CAS
北大核心
2020年第10期1260-1267,共8页
Chemical Reagents
基金
吉林省科技厅重点科技研发项目(2018-0201073YY)
吉林化工学院重大研发项目(吉化院合字[2015]第005号)。
关键词
改良索氏提取器
人参须根皂苷
人参皂苷RG3
制备
生成路径
柠檬酸
improved soxhlet extractor
Fibrous Root Ginsenoside
ginsenoside Rg3
preparation
production pathway
citric acid