摘要
More than 32,000 pathogenic single nucleotide polymorphisms(SNPs)have been identified in the human genome(Gaudelli et al.,2017).Genetically modified mice with pathogenic SNPs are good models for studies of disease pathogenesis and the development of new therapeutics.Accordingly,an efficient,high-throughput method for the generation of mouse models with SNPs is needed.
基金
supported by the National Key R&D Program of China(2017YFC1001901,2017YFA0102801 and 2017YFC1001603)
the National Natural Science Foundation of China(91640119,31671540,81330055 and 31601196)
the Guangdong Special Support Program(2019BT02Y276)
the Natural Science Foundation of Guangdong Province(2016A030310206 and 2014A030312011)
the Science and Technology Planning Project of Guangdong Province(2015B020228002)
the Guangzhou Science and Technology Project(201707010085 and 201803010020)。
