miR-1182在卵巢癌组织中的表达及对肿瘤细胞增殖与转移的影响

Expression of miR-1182 in ovarian cancer and its effect on tumor cell proliferation and metastasis

目的探讨miR-1182在卵巢癌组织中的表达及对肿瘤细胞增殖与转移的影响。方法使用反转录聚合酶链式扩增(RT-PCR)技术检测正常卵巢组织和卵巢癌及相应癌旁组织中的miR-1182表达水平。用miR-1182 mimics病毒转染人卵巢癌细胞株SKOV-3,并用RT-PCR和Western Blot检测细胞的miR-1182和hTERT蛋白的表达水平。用噻唑兰法检测转染后的SKOV-3细胞的增殖与侵袭情况。结果卵巢癌组织中miR-1182的表达水平明显低于正常卵巢组织与癌旁组织(均P<0.05)。对于miR-1182表达水平,不同肿瘤分期、组织分化程度的患者间的差异有统计学意义(均P<0.05),但患者年龄与肿瘤类型间的差异无统计学意义(均P>0.05)。与空白病毒组和对照组相比,转染miR-1182后,转染组SKOV-3细胞的miR-1182表达水平明显升高(均P<0.05),而hTERT蛋白表达明显降低(均P<0.05)。转染组SKOV-3细胞在转然后24、48、72 h细胞增殖和迁移能力受到明显抑制(均P<0.05)。结论miR-1182在卵巢癌组织中低表达,过表达miR-1182可抑制卵巢癌的增殖与侵袭能力,其可能通过靶向调节hTERT的表达实现调控作用。

Objective To investigate the expression of miR-1182 in ovarian cancer tissues and its effect on tumor cell proliferation and metastasis.Methods Reverse transcription polymerase chain amplification(RT-PCR)technology was used to detect the expression level of miR-1182 in normal ovarian tissue,ovarian cancer and corresponding adjacent tissues.The human ovarian cancer cell line SKOV-3 was transfected with miR-1182 mimics virus,and the expression levels of miR-1182 and hTERT protein were detected by RT-PCR and Western Blot respectively.The thiazolyl method was used to detect the proliferation and invasion of the transfected SKOV-3 cells.Results The expression level of miR-1182 in ovarian cancer tissue was significantly lower than that in normal ovarian tissue and adjacent tissues(all P<0.05).Regarding the expression level of miR-1182,the difference between patients with different tumor stages and degree of tissue differentiation was statistically significant(all P<0.05),but the difference between patient age and tumor type was not statistically significant(all P>0.05).Compared with the blank virus group and the control group,the miR-1182 expression level of the transfected group was significantly increased(all P<0.05),while the expression of hTERT protein was significantly decreased(all P<0.05).The proliferation and migration ability of SKOV-3 cells in the transfected group was significantly inhibited at 24,48,and 72 h after transfection(all P<0.05).Conclusions The expression of miR-1182 is low in ovarian cancer tissues.Overexpression of miR-1182 can inhibit the proliferation and invasion of ovarian cancer,and the mechanism may be related to the targeted regulation of hTERT expression to achieve regulation.