带有孔隙硅胶鼻假体对兔包膜形态的影响

Effect of pores of silicone nasal implant on morphology of surrounding capsule

目的带有孔隙的硅胶假体植入兔软硬组织之间,观察假体周边包膜的形态学变化。方法2018年9月在大连大学动物实验中心将规则排列微孔的硅胶鼻假体切割成1 cm硅胶段,植入5只1年雌性新西兰大耳白兔前额骨膜下。3个月后,将假体及其周边的组织取出行组织病理检查,β-catenin和Vimentin免疫组织化学染色观察。结果肉眼可见包膜较薄,透过包膜可见白色假体。假体小孔处颜色较深,显示组织较厚,撕脱时有阻力;撕脱包膜内可见规律突起,小于假体表面小孔。组织病理可见实验样本小孔内全部有组织长入,假体周边形成菲薄包膜;包膜在小孔内及小孔间连续;未见异物巨噬细胞。孔内组织有的含有较多纤维,有的为疏松结缔组织,与皮下疏松结缔组织相延续。β-catenin、Vimentin免疫组织化学染色包膜阳性,包膜内组织染色阴性,包膜深面组织散在细胞胞质Vimentin染色阳性。结论带有孔径的硅胶假体植入兔体后,假体周边形成包膜;包膜对应假体小孔处形成小突起,突起为包膜包裹的正常组织。

Objective To observe the morphologic changes of the tissue growing into the prefabricated pores on the surface of the silicone nasal implant.Methods The silicone nasal implant with regularly arranged holes was cut into 1 cm-long segments and inserted to the forehead subperiosteal pockets of five female New Zealand white rabbits(1-year old)in the Animal Experiment Center of Dalian University.Three months later,the implant and its surrounding tissues were removed and observed after HE staining,β-catenin and vimentin immunohistochemical staining.Results The capsule and its implant were visible to the naked eyes.The capsule was thin,and the white implant was seen through the capsule.The small pore of the implant showed darker color,indicating that the tissue here was thicker.The capsule was cut open and the capsule was torn off the implant.It could obviously be felt the resistance of the tissue in the small pores to the external pulling.Regular protrusions were found on the inner surface of the peeled capsule.No infection occurred in the surgery sites.Under the HE staining microscope,it was found that 100%pores in all the experimental samples were filled with the tissue.The thin capsule was formed around the implant.The capsule was continuously intact in and between the pores.No macrophages were seen.Some of the intracapsular tissues contained more fibers,and some were loose connective tissue.The immunohistochemical staining ofβ-catenin and vimentin showed that the tissue in the capsule was negative.Vimentin staining for the cytoplasmic scattered cells in the deep capsule tissue was positive.Conclusions Based on the results of the experiments,it is concluded that the tissue can grow into the small pores of the implant.The capsules around the implant,which are smooth with protrusions,are connected on the surface of the implant and at the bottom of the pores.The protrusion pointing to the pores of implant is normal tissue covered by thin capsule.