ZT和IBA浓度对5个蓝莓品种离体增殖的影响

Effect of concentrations of ZT and IBA on multiplication in vitro of five blueberry varieties

以5个蓝莓品种(‘蓝丰’‘早蓝’‘齐佩瓦’‘密斯提’和‘夏普蓝’)的试管苗茎段为试验材料,研究了ZT和IBA不同浓度配比对5个蓝莓品种试管苗增殖的影响。结果表明,在WPM培养基中,蓝莓品种不同,其试管苗增殖适宜的ZT和IBA浓度不同,添加ZT 1.0 mg/L对‘蓝丰’增殖效果最佳,增殖系数为3.41,试管苗高为3.0 cm;添加ZT 1.5 mg/L对‘早蓝’的增殖效果最佳,增殖系数为3.60,苗高为4.1cm;添加ZT 1.5 mg/L+IBA 0.05 mg/L对‘密斯提’的增殖效果最佳,增殖系数为2.71,苗高为4.8 cm;添加ZT 2.0 mg/L+IBA 0.05 mg/L对‘齐佩瓦’和‘夏普蓝’增殖效果最佳,增殖系数分别为4.32和6.70,苗高分别为4.9 cm和3.5 cm。综合分析得出,‘蓝丰’的最佳离体茎段增殖培养基为WPM+ZT 1.0 mg/L;‘早蓝’的最佳离体茎段增殖培养基为WPM+ZT 1.5 mg/L;‘密斯提’的最佳离体茎段增殖培养基为WPM+ZT 1.5 mg/L+IBA 0.05 mg/L;‘齐佩瓦’和‘夏普蓝’的最佳离体茎段增殖培养基为WPM+ZT 2.0 mg/L+IBA 0.05 mg/L。

The effects of different concentrations of ZT and IBA on the multiplication of 5 blueberry varieties(Bluecrop,Earliblue,Chippewa,Misty and Sharpblue)seedlings in vitro were studied by using the stem segments in vitro of 5 blueberry varieties as materials.The results showed that the different varieties of blueberry had different appropriate ZT and IBA concentrations for seedling multiplication in vitro when they were cultured in WPM medium.Adding ZT 1.0 mg/L had the best effect on the multiplication of Bluecrop,with a multiplication coefficient of 3.41 and plant height of 3.0 cm.Adding ZT 1.5 mg/L had the best effect on multiplication of Earliblue,with a multiplication coefficient of 3.6 and plant height of 4.1cm.Adding ZT 1.5 mg/L+IBA 0.05 mg/L had the best effect on multiplication of Misty,with a multiplication coefficient of 2.71 and plant height of 4.8 cm.Adding ZT 2.0 mg/L+IBA 0.05 mg/L had the best effect on the multiplication of Chippewa and Sharpblue,with multiplication coefficients of 4.3 and 6.7,respectively,and plant height of 4.9 cm and 3.5 cm,respectively.Through comprehensive analysis,the optimal medium was WPM+ZT 1.0 mg/L for Bluecrop stem multiplication in vitro,WPM+ZT 1.5 mg/L for Earliblue,WPM+ZT 1.5 mg/L+IBA 0.05 mg/L for Misty and WPM+ZT 2.0 mg/L+IBA 0.05 mg/L for Chippewa and Sharpblue.