PEG修饰Fe3O4纳米粒子(MNPs)的制备及载药研究

Preparation and drug loading of PEG modified Fe3O4 nanoparticles

盐酸阿霉素(DOX)是一种抗肿瘤抗生素,通过抑制癌细胞遗传物质核酸的合成发挥作用,其抗瘤谱较广,对于多种肿瘤细胞均有杀灭作用。本研究首先通过共沉淀合成法制备Fe3O4纳米粒子(MNPs),再依法制备Fe3O4@SiO2以及Fe3O4@SiO2-NH2纳米粒子,然后对Fe3O4@SiO2-NH2样品进行单端羧基PEG和双端羧基PEG的修饰,得到Fe3O4@SiO2-NH2-PEG和Fe3O4@SiO2-NH2-PEGCOOH样品,利用PEG修饰产物装载不同质量的盐酸阿霉素(DOX),得到Fe3O4@SiO2-NH2-PEG·DOX和Fe3O4@SiO2-NH2-PEG-COOH·DOX。荧光分光光度计检测结果表明,PEG修饰的Fe3O4纳米粒子(MNPs)载入不同质量的DOX除增加阿霉素的磁靶向性能外,因PEG的引入,生物相容性也得到增强。

Doxorubicin hydrochloride(DOX)is an anti-tumor antibiotic that acts by inhibiting the synthesis of nucleic acid in cancer cells.DOX has a broad anti-tumor spectrum and has a killing effect on a variety of tumor cells.In this paper,Fe3O4 nanoparticles(MNPs)were prepared by co-precipitation synthesis method,while Fe3O4@SiO2 and Fe3O4@SiO2-NH2 nanoparticles were prepared by optimizing experimental conditions.And then single and double carboxyl PEG were applied to modify Fe3O4@SiO2-NH2 samples.Fe3O4@SiO2-NH2-PEG and Fe3O4@SiO2-NH2-PEG-COOH samples could be obtained.PEG modified products were loaded with different quality of DOX.Fe3O4@SiO2-NH2-PEG·DOX and Fe3O4@SiO2-NH2-PEG-COOH·DOX samples could be obtained.After detection by a fluorescence spectrophotometer,a conclusion was drawn.The results showed that PEG-modified Fe3O4 nanoparticles(MNPs)loaded with different masses of DOX could increase the magnetic targeting properties of DOX,at the same time,biocompatibility could also be enhanced via the introduction of PEG.