摘要
盐酸阿霉素(DOX)是一种抗肿瘤抗生素,通过抑制癌细胞遗传物质核酸的合成发挥作用,其抗瘤谱较广,对于多种肿瘤细胞均有杀灭作用。本研究首先通过共沉淀合成法制备Fe3O4纳米粒子(MNPs),再依法制备Fe3O4@SiO2以及Fe3O4@SiO2-NH2纳米粒子,然后对Fe3O4@SiO2-NH2样品进行单端羧基PEG和双端羧基PEG的修饰,得到Fe3O4@SiO2-NH2-PEG和Fe3O4@SiO2-NH2-PEGCOOH样品,利用PEG修饰产物装载不同质量的盐酸阿霉素(DOX),得到Fe3O4@SiO2-NH2-PEG·DOX和Fe3O4@SiO2-NH2-PEG-COOH·DOX。荧光分光光度计检测结果表明,PEG修饰的Fe3O4纳米粒子(MNPs)载入不同质量的DOX除增加阿霉素的磁靶向性能外,因PEG的引入,生物相容性也得到增强。
Doxorubicin hydrochloride(DOX)is an anti-tumor antibiotic that acts by inhibiting the synthesis of nucleic acid in cancer cells.DOX has a broad anti-tumor spectrum and has a killing effect on a variety of tumor cells.In this paper,Fe3O4 nanoparticles(MNPs)were prepared by co-precipitation synthesis method,while Fe3O4@SiO2 and Fe3O4@SiO2-NH2 nanoparticles were prepared by optimizing experimental conditions.And then single and double carboxyl PEG were applied to modify Fe3O4@SiO2-NH2 samples.Fe3O4@SiO2-NH2-PEG and Fe3O4@SiO2-NH2-PEG-COOH samples could be obtained.PEG modified products were loaded with different quality of DOX.Fe3O4@SiO2-NH2-PEG·DOX and Fe3O4@SiO2-NH2-PEG-COOH·DOX samples could be obtained.After detection by a fluorescence spectrophotometer,a conclusion was drawn.The results showed that PEG-modified Fe3O4 nanoparticles(MNPs)loaded with different masses of DOX could increase the magnetic targeting properties of DOX,at the same time,biocompatibility could also be enhanced via the introduction of PEG.
作者
辛亮亮
舒玥
刘欢
胡雅稳
杨琳燕
XIN Liang-liang;SHU Yue;LIU Huan;HU Ya-wen;YANG Lin-yan(Domestic Discovery Service Unit,Wuxi App Tec Co.,Ltd.,Tianjin 300457,China;Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300384,China)
出处
《天津农学院学报》
CAS
2020年第3期43-48,共6页
Journal of Tianjin Agricultural University
基金
天津农学院杰出科研业绩奖励资助项目(无编号)
国家自然科学基金(31572492,31372482)
省部共建药用资源化学与药物分子工程国家重点实验室资助课题(CMEMR2016-B12)。