超高压法提取葛根多糖的工艺研究

Study on the technology of extracting polysaccharides from Radix Puerariae by Ultra-high pressure method

本文以葛根块为原料,采用超高压技术提取葛根中的多糖,以多糖提取率为评价指标,考察超高压压力水平、保压时间、料液比、浸泡时间对葛根多糖提取率的影响,通过单因素和正交试验对葛根多糖的超高压提取工艺方法进行优化,确定最佳的提取工艺条件为:料液比1∶50(g/mL)、超高压压强300 MPa、保压时间3 min、浸泡时间90 min。在此条件下,超高压法提取葛根多糖的提取率为23.81 mg/g。

In this paper,Ultra-high pressure technology(UHP)was used to extract polysaccharide from Radix Puerariae with the extraction rate of polysaccharide as the evaluation index.UHP pressure level,UHP time,solid-liquid ratio and soaking time were analyzed.Through single factor and orthogonal test,the optimal extraction conditions were obtained:liquid ratio 1∶50(g/mL),UHP pressure 300 MPa,UHP time 3 min,soaking time 90 min.Under these conditions,ultra high pressure extraction rate of extraction of polysaccharide was 23.81 mg/g.