摘要
Background:This study aims to observe the effects of Yishenbupi(Tonifying-Kidney and Invigorating-Spleen)decoction on renal fibrosis of unilateral ureteral occlusion rats.Methods:Forty-eight sprague-dawley rats were randomly divided into the sham-operated group(sham group),model group,irbesartan group,and Yishenbupi group.Each group was intragastrically administered after the unilateral ureteral occlusion model was established.Rats in the Yishenbupi group were intragastrically administered with Yishenbupi decoction(18 g/kg/d)once every morning.Rats in the irbesartan group were intragastrically administered with 10 mg/kg/d of irbesartan tablets once every morning.Rats in the sham group and model group(unilateral ureteral occlusion group)were intragastrically administered with isovolumetric distilled water twice a day from the day the model was established.All rats were sacrificed 21 days later.Occluded kidney tissues were taken,and pathological sections were prepared.Masson,periodic acid-Schiff,Sirius Red,and immunohistochemical staining were performed to detect the expression of collagen III and fibronectin.Results:The pathological staining of rat kidneys(Masson,periodic acid-Schiff,and Sirius Red)showed that,compared to the unilateral ureteral occlusion model group,the renal interstitial injury was eased and collagen deposition was reduced in the irbesartan and Yishenbupi groups;after immunohistochemical staining,the expression of collagen III and fibronectin positively expressed cells was decreased and decreased more in the Yishenbupi group than in the irbesartan group.Conclusion:Yishenbupi decoction can alleviate the injury to kidney tissues in rats with unilateral ureteral obstruction,reduce the deposition of extracellular matrix,and act against renal fibrosis.
基金
This study was supported by National Natural Science Foundation of China(No.81460719)
Guangxi Natural Science Foundation(No.2017GXNSFAA198217).
