黄麻SSR标记与纤维产量性状的相关性

Correlation between SSR markers and fiber yield related traits in jute(Corchorus spp.)

鉴定出与纤维产量相关性状连锁的SSR标记有助于黄麻纤维产量的分子标记辅助育种。本研究以311份黄麻种质资源为研究材料,通过2016—2018年表型鉴定,结合116对SSR引物扩增出397个SSR标记,利用SPSS软件对SSR标记与纤维产量性状进行相关性分析。结果表明, 311份黄麻种质资源9个纤维产量性状的变异系数范围为13.05%~76.78%,表现出广泛的遗传变异。农艺性状相关分析表明,这些性状间存在极显著相关,其中分枝高和节数的平均相关系数最高(r=0.931),其次是单株鲜皮重和单株鲜茎重(r=0.781)、单株干皮重与单株鲜皮重(r=0.779)。方差分析表明,节数、分枝数、株高以及分枝高等性状在不同年份间相对稳定,广义遗传力较高。进而通过皮尔逊相关法鉴定与纤维产量性状相关的SSR标记。每个性状与这些相关SSR标记逐步回归分析表明,与纤维产量性状显著相关的标记有6个,单个SSR标记的贡献率为3.9%~22.5%。这些结果将会加快黄麻设计育种进程。

Simple sequence repeat(SSR)markers correlated to fiber yield traits would be a beneficial tool for molecular marker-assisted breeding in jute.In this study,397 SSR markers were screened from 311 jute germplasms by the phenotype identification from 2016 to 2018 and 116 pairs of primers.The correlation analysis between SSR markers and fiber yield related traits by SPSS revealed that the range of the coefficient of variation related to 9 fiber traits was from 13.05%to 76.78%,indicating a comprehensive genetic variation.The correlation analysis of the agronomic traits showed that there were significantly correlations among these traits.The average correlation coefficient between branch height and nodes of main stem was the highest(r=0.931),followed by the correlation coefficients(r=0.781)of fresh bark weight per plant and fresh stem weight per plant and the coefficients(r=0.779)of dry bark weight and fresh bark weight per plant.Analysis of variance(ANOVA)showed that the traits of main stem nodes,number of branches,plant height and branch height were relatively stable in different years with the higher broad heritability capacity.Furthermore,the SSR markers associated with fiber yield related traits were identified by Pearson correlation method.Stepwise regression analysis among each trait associated SSR markers indicated that there were six markers associated significantly with fiber yield related traits,and phenotypic variation explained by each SSR marker varied from 3.9%to 22.5%.These results will accelerate the development of molecular design breeding in jute.