Trametes sp.LS-10C固态发酵产漆酶培养基优化及其对双酚A的降解

Optimization of laccase production by Trametes sp.LS-10C under solid-state fermentation and bisphenol A degradation by the laccase product

漆酶是一种绿色高效的多酚氧化酶,在降解双酚A方面具有巨大潜力。为降低发酵产漆酶的成本及考察漆酶在双酚A降解中的能力,本研究以麸皮和柚皮为主要基质,优化了栓菌固态发酵产漆酶条件,对优化后获得的漆酶在双酚A降解中的应用进行了研究。结果表明,在培养基组分为:麸皮和柚皮粉比例为6:4(W/W)、固液比1:2.5(W/V)、铜离子2%(W/W)、蔗糖3%(W/W)、硝酸钾2%(W/W)、稻壳20%(W/W)的条件下,栓菌发酵产漆酶的酶活最高,发酵11d后,酶活可达到38.4U/gds。当双酚A初始浓度为10μg/m L时,在55℃条件下酶解140min后,双酚A基本降解完全。

Laccase is a green and efficient oxidase for biodegradation of polyphenol,which has a great potential in the degradation of bisphenol A.For reducing the production cost of laccase,the solid-state fermentation conditions for Trametes sp.were optimized by using bran and grapefruit peel as the main substrates,and the degradation ability of the laccase product to bisphenol A was further investigated.As a result,by using the optimized medium containing components formulated in the proportion of bran and grapefruit peel ratio of 6:4(W/W),solid-liquid ratio of 1:2.5(W/V),copper ion 2%(W/W),sucrose 3%(W/W),potassium nitrate 2%(W/W),and rice husk 20%(W/W),the enzyme activity of the laccase product reached 38.4 U/gds in 11 days of fermentation.Under initial concentration of 10μg/mL,bisphenol A was almost completely degraded by laccase at 55℃within incubation duration of 140 min.