16S rDNA测序分析微酸性电解水对牙周炎患者口腔菌群的影响

Effect of Slightly Acidic Electrolyzed Water on Oral Flora in Patients with Periodontitis by 16S rDNA Sequencing Analysis.

目的:探讨微酸性电解水含漱液对牙周炎患者口腔菌群的影响。方法:选择10例进入支持治疗的牙周炎患者,收集其使用微酸性电解水含漱7 d前、后的唾液样本,通过16S rDNA测序法对样本进行检测,分析口腔菌群的变化。结果:用微酸性电解水含漱7 d后,门水平,拟杆菌门、梭菌门、变形菌门物种相对丰度降低。属水平,奈瑟菌属、梭杆菌属、卟啉单胞菌属、普雷氏菌属物种相对丰度降低。α多样性分析:Simpson指数增加,Shannon指数增加(P<0.05),Chao1指数、ACE指数、PD_whole_tree指数均升高(P<0.01)。β多样性指数分析显示组间差异性显著(P<0.01)。结论:含漱微酸性电解水可以改变口腔菌群的结构,增加菌群种类及多样性。

Objective:To investigate the effect of slightly acidic electrolyzed water(SAEW)gargle on oral flora in patients with periodontitis.Methods:The saliva samples gargled with slightly acidic electrolyzed water of 10 patients with periodontitis who entered the supportive periodontal therapy were collected before and after 7 days.The total DNA of oral flora was extracted and sequenced by 16S rDNA sequencing,and the changes of oral flora diversity and structure were analyzed.Results:After gargled with slightly acidic electrolyzed water for 7 days,at phylum level,the relative abundance of proteobacteria,bacteroidetes and fusobacteria species was decreased.At the genus level,the relative abundance of periodontal pathogen genera-neisseria,porphyromonas,clostridium,and pretenella was decreased.Analysis ofα-diversity:the Simpson index and Shannon index increased(P<0.05),Chao1 index,ACE index,and PD_whole_tree index were all increased(P<0.01).The analysis of theβ-diversity index showed significant differences between the groups(P<0.01).Conclusion:The use of slightly acidic electrolyzed water can change the structure of oral flora and increase the abundance and diversity of oral flora in patients with periodontitis,which is conducive to the restoration of oral flora to the normal range.