马赛菌属Massilia neuiana异养硝化-好氧反硝化脱氮特征实验

ANALYSIS ON THE HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFICATION CHARACTERISTICS OF THE NEW SPECIES OF MASSILIA NEUIANA

基于马赛菌属脱氮Massilia neuiana的模式菌株PTW21进行研究,探讨其异养硝化-好氧反硝化脱氮能力。菌株PTW21具有高效异养硝化和好氧反硝化能力,对NH^+4-N和NO^-2-N去除率均超过90%。同时,菌株PTW21具有同步硝化反硝化能力,且反硝化效率高于硝化效率,但当有NH^+4-N存在时,会优先利用NH^+4-N,再利用NO^-2-N,存在硝化-反硝化竞争抑制现象。同步硝化反硝化时,菌株PTW21可以去除95%以上的NH^+4-N和NO^-2-N。Massilia neuiana的研究丰富了异养硝化-好氧反硝化微生物的种类,也为该菌种在污水处理厂的生物强化应用提供了前期基础。

In this study,the heterotrophic nitrification-aerobic denitrification characteristics of strain PTW21,a typical strain of a novel nitrogen removal species Massilia neuiana,was investigated.The high efficiencies of heterotrophic nitrification and aerobic denitrification by strain PTW21 were demonstrated,which were both obtained over 90%.A higher denitrification efficiency was obtained than its nitrification efficiency in the simultaneous nitrification and denitrification(SND)by strain PTW21.However,a nitrification-denitrification competitive inhibition was observed in the presence of NH^+4-N that ammonium was preferentially consumed by strain PTW21,followed by nitrate.Totally over 95%of ammonia and nitrate nitrogen were removed by strain PTW21 in the SND process.The study on Massilia neuiana enriched the variety of heterotrophic nitrification-aerobic denitrification microbe,and established the preliminary foundation of the strain for the bio-augmentation application in sewage treatment plants.