CRISPR/Cas9在清除潜伏HIV前病毒基因组中的研究进展

Research progress of CRISPR/Cas9 in eliminating latent HIV previrus genome

抗反转录病毒疗法(ART)是目前治疗艾滋病(AIDS)的首选方法,因无法清除整合到CD4+T细胞染色体上的艾滋病病毒(HIV)前病毒基因,在停止ART治疗后,整合的HIV前病毒基因会随着T细胞的活化被激活,从而重新复制感染,因此HIV感染者/AIDS患者须终生使用抗病毒药物。成簇规律间隔短回文重复序列及其核酸酶Cas9(CRISPR/Cas9)系统,因其设计简单、操作方便,且无种属间限制,成为最具有发展前景的基因组定点编辑技术。本文就CRISPR/Cas9系统及消除潜伏的HIV前病毒基因中的研究进展进行综述。

Antiretroviral therapy(ART)is currently the preferred treatment for HIV/AIDS.However,it cannot remove the integrated viral DNA(provirus)on CD4^+T,and patients with HIV/AIDS must have lifelong use of antiviral drugs.After discontinuation of ART,the integrated viral DNA is activated with the activation of T cells,thereby to replicate the infection.Cluster regular interval short palindrome repeat sequence and its nuclease Cas9(CRISPR/Cas9)system are not only simple in design and easy operation,but also have no inter-species restrictions,thus becoming the most promising fixed-point genome editing technology.This paper reviews the CRISPR/Cas9 system and its progress in elimination of latent HIV previrus genes.