山西老陈醋源植物乳杆菌CP234对大鼠非酒精性脂肪肝的保护作用及机制

Protective Effect of Lactobacillus plantarum CP234 Isolated from Shanxi Aged Vinegar on Nonalcoholic Fatty Liver Disease in Rats

为研究山西老陈醋源植物乳杆菌CP234对大鼠的非酒精性脂肪肝的保护作用和机制,对大鼠灌胃植物乳杆菌CP234,结果显示:高脂饮食大鼠血清中的总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和甘油三酯(TG)的含量显著降低,改善了肝脏脂肪酸的组成。植物乳杆菌CP234还通过降低血清中谷丙转氨酶(ALT)和谷草转氨酶(AST)的活性,提高肝脏中谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)的酶活性,改善大鼠肝脏的氧化应激能力,进而修复肝功能。荧光定量PCR结果显示:连续灌胃植物乳杆菌CP2345周后,相比于模型组,胆固醇分解、脂肪酸氧化的相关基因显著上调,而PPAR-γ、FAS和ACC-1等与脂肪合成基因的表达显著下调。本文还测定了植物乳杆菌CP234对高脂饮食大鼠肠道菌群和粪便有机酸、pH值、水分的影响,结果显示其通过调节肠道菌群平衡改善肠道内环境,从而防治非酒精性脂肪肝疾病。

The protective effect of Lactobacillus plantarum CP234 isolated from Shanxi aged vinegar on nonalcoholic fatty liver disease(NAFLD)in rats were investigated.Oral administration of L.plantarum CP234 significantly decreased the levels of serum total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C)and triglyceride(TG)in rats with high fat diet induced NAFLD.Also,the lipid profile of liver were significantly changed.Serum alanine transaminase(ALT)and aspartate aminotransferase(AST)were decreased,meanwhile,superoxide dismutase(SOD)and glutathion reductase(GSH)in liver were enhanced,which recovered and improved the oxidative stress ability and function of liver.The fluorescence quantitative PCR results suggested that downregulating lipogenesis(PPAR-γ、FAS and ACC-1)and upregulating genes expression related to lipolysis were also involved in the beneficial effect of L.plantarum CP234 on NAFLD.Furtherly,the effect of L.plantarum CP234 on intestinal floras and fecal pH and water content were studied.