摘要
通过形态学观察、16S rRNA序列分析、MALDI-TOF质谱分析、VITEK 2 COMPACT全自动微生物分析、API试剂条方法,对GB 4789.28-2013《食品安全国家标准食品微生物学检验培养基和试剂的质量要求》中指定使用的培养基质控菌株CMCC(B)40001进行多相复核鉴定,确定其分类学地位。结果表明:CMCC(B)40001为支气管炎鲍特氏菌,与GB 4789.28-2013收录的名称粪产碱杆菌不一致。
To determine the taxonomic classification of reference strain CMCC(B)40001,which cited in GB 4789.28-2013 National food safety standard Food microbiological examination:Quality requirements of medium and reagents,polyphasic identification approaches including morphological features,16s rRNA sequence,MALDI-TOF MS,VITEK 2 COMPACT automatic microbial analysis system and API were used.Results indicated that CMCC(B)40001 was identified as Bordetella bronchiseptica and inconsistent with the scientific name-Alcaligenes faecalis in GB 4789.28-2013.
作者
都海渤
刘佳奇
张欣
李婷
李金霞
姚粟
Du Haibo;Liu Jiaqi;Zhang Xin;Li Ting;Li Jinxia;Yao Su(China National Research Institute of Food and Fermentation Industries Corporation Limited,China Center of Industrial Culture Collection,Beijing 100015)
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2020年第11期273-278,共6页
Journal of Chinese Institute Of Food Science and Technology
基金
国家菌种资源库工业菌种资源分库运行与服务项目(NMRC-2019-4)。
