基于系统药理学探讨辣椒素潜在作用靶点的研究

Potential Targets of Capsaicin Based on System Pharmacology

[目的]系统性探讨辣椒素的潜在靶点及其作用。[方法]以系统药理学方法为手段,首先用不同的计算机靶点预测方法预测辣椒素的潜在靶点,接着用GeneMANIA进行基因富集分析,然后借助DAVID数据库对预测靶蛋白进行基因本体GO富集分析和疾病富集分析,并利用Cytoscape 3.4软件构建辣椒素潜在的作用网络。[结果]辣椒素可作用于25个潜在靶蛋白;它们的相互作用主要是通过预测、蛋白共表达、物理相关性、共享蛋白域和共定位等表现出来;GO分析有11个条目;疾病富集发现辣椒素与肿瘤、药物超敏、代谢病和炎症等多种疾病密切相关;构建了构建“辣椒素-靶点-疾病”网络。[结论]辣椒素是通过多靶点作用于多种疾病的,该研究为其食用安全性和功能性评价提供了理论依据。

[Objective]To investigate the potential targets and their functions of capsaicin.[Method]In this study,firstly,the potential targets of capsaicin were predicted by different computer target prediction methods.Then,for all predicted targets,gene enrichment was analyzed by GeneMANIA,and then the gene ontology(GO)enrichment and disease enrichment were analyzed by DAVID database.At last,the potential action network of capsaicin was constructed by Cytoscape 3.4.[Result]It was found that capsaicin can act on 25 potential target proteins.Their interactions of target proteins were mainly manifested by prediction,co-expression,physical interactions,shared protein domains and co-localization.GO analysis had 11 items.Disease enrichment showed that capsaicin is closely related to various diseases such as tumors,drug hypersensitivity,metabolic diseases and inflammation.[Conclsion]Capsaicin is a multi-targeted agent on a variety of complex diseases,this study provided a theoretical basis for its food safety and functional evaluation.