人和猪神经脱细胞基质蛋白质组学差异

Proteomic analysis by a comparison in protein difference between porcine and human decellularized nerve matrices using

目的:分析猪与人外周神经在脱细胞后的蛋白质组学差异,为猪神经脱细胞材料运用于外周神经损伤(PNI)的修复提供一定的数据支撑。方法:通过研磨和酶解方式提取猪神经脱细胞基质(pDNM)和人神经脱细胞基质(hDNM)内的所有蛋白,采用以串联质量标签(TMT)为标记的液相色谱质谱联用(TMTHPLC-MS/MS)技术定量并对2组差异蛋白进行GO富集化和KEGG通路分析。结果:共鉴定1448种蛋白,组间蛋白都能明显区分,组内蛋白相关性良好。其中,pDNM上调蛋白数456个,下调蛋白数170个,且pDNM富含更多的细胞外基质(ECM)相关蛋白。GO分析显示,上调表达蛋白所处的细胞位置集中在生长锥、轴突、神经元胞体。上调表达蛋白参与的生物学过程为细胞迁移、细胞骨架组织、轴突发育。上调蛋白的主要分子功能与生物功能调节、细胞骨架的结构组成、肌动活性有关。KEGG通路分析显示,差异蛋白可参与肌动蛋白细胞骨架的调节、轴突的引导等信号通路的激活。结论:pDNM内含丰富的结构和功能相关蛋白,有望成为修复PNI的备选产品。

Objective:To analyze the proteomic differences between porcine and human peripheral nerves after acellularization,which will provide the porcine decellularizedmaterial as a promising platformfor repairing peripheral nerve injury(PNI).Methods:All proteins in the porcine decellularized nerve matrix(pDNM)and human decellularized nerve matrix(hDNM)were extracted by grinding and enzymolysis.Then,the differentially expressed proteins(DEPs)were done with GO and KEGG pathway analysis using TMT labeled nano-HPLC-MS/MS technology.Results:A total of 1448 corresponding proteins were identified,of which 456 and 170 proteins in pDNM manifested respective upregulation and downregulation,when compared with hDNM.Moreover,pDNM also contained more extracellular matrix(ECM)related proteins.GO functional annotation results suggested that the upregulated expressing proteins involved GO terms for cellular component were commonly enrichedin growth cone,axon and neuronal cell body;involved GO terms for biological process were generally associated with cell migration,cytoskeleton organization and axon development;andinvolved GO terms for molecular function were mainly related with molecular function regulator,structural constituent of cytoskeleton and motor activity.KEGG analysis also revealed that most of these DEPs participated in the regulation of actin cytoskeleton,axon guidance and ECM-receptor interaction.Conclusion:pDNM,thanks to its abundant structural and functional proteins,is a potential optional substitute for repairing PNI.