快速病原体检测技术在甲型流感病毒检测中的应用

Clinical application of rapid pathogen detection technologies for detecting influenza A virus

目的探究甲型流感病毒(FluA)抗原检测法、核酸检测法和快速病原体检测技术的临床应用价值。方法收集2016年10月—2019年1月西京医院临床疑似FluA感染患者鼻咽拭子样本,分别采用FluA核酸检测法、抗原检测法进行检测,比较2种检测方法的结果,评价快速呼吸道病原体检测技术的临床应用价值。结果FluA抗原检测法的阳性检出率为9.56%,FluA核酸检测法的阳性检出率为27.29%。每年12月—次年2月为FluA感染暴发季,其抗原筛查阳性率为10.95%,核酸检测阳性率为27.71%;<5岁的婴幼儿、约20岁的青少年以及>90岁的老年人为易感人群;男性与女性比较,FluA核酸检测结果差异无统计学意义(P<0.05)。快速呼吸道病原体检测检出阳性170例,阳性结果207个,其中部分患者为多种呼吸道病原体混合感染。结论抗原和核酸检测均能较好地初步筛查FluA感染,快速病原体检测技术可快速检出多种常见呼吸道感染相关病原体,对临床呼吸道感染患者的诊断和治疗有重要意义。

Objective To investigate the clinical application roles of influenza A virus(FluA)antigen,FluA nucleic acid and Filmarray rapid pathogen detection technologies for detecting FluA.Methods The patients with suspected FluA infection in Xijing Hospital from October 2016 to January 2019 were enrolled for the detections of FluA nucleic acid and antigen.The results were analyzed comparatively.Filmarray rapid pathogen detection was used as well.Results The positive rate of FluA antigen detection was 9.56%,and the positive rate of FluA nucleic acid detection was 27.29%.The period from December to February was the outbreak season,and the positive rate of FluA antigen detection in this period was 10.95%,and the positive rate of FluA nucleic acid detection in this period was 27.71%.Infants<5 years old,adolescents around 20 years old and elders>90 years old were susceptible subjects.There was no statistical significance in FluA nucleic acid detection between males and females(P<0.05).Using Filmarray rapid pathogen detection technology,170 positive specimens were detected,and 207 positive results were obtained,some of which included multiple infection resulted from different respiratory pathogens.Conclusions FluA antigen and nucleic acid detections could clarify the infection.Filmarray rapid pathogen detection technology could serve the diagnosis of infectious diseases and is of significance for the diagnosis and treatment of clinical respiratory tract infection.