益气解毒方对2.0 Gy60Co γ射线致小鼠睾丸急性损伤的防护效应

Preventive effects of Yiqi Jiedu Fang on acute testicular injury of mice induced by 2.0 Gy Co γ ray

目的观察益气解毒方对2.0 Gy 60Coγ射线致雄性Balb/c小鼠睾丸急性损伤的防护效应。方法将200只雄性Balb/c小鼠按体重以随机区组方法分为正常组、模型组、安多霖组和益气解毒方高、低剂量组。除正常组外,其余小鼠均给予2.0 Gy 60Coγ射线一次性全身照射。各给药组小鼠于照前连续10 d给予相应药物,正常组及模型组小鼠同步灌胃去离子水。各组小鼠分别在照前1 d和照后1、3、7 d取材,检测小鼠体重、睾丸指数、睾丸组织形态学变化以及生精细胞凋亡情况。结果与正常组比较,照前1 d各组小鼠检测指标均无显著性差异;照后1 d,模型组小鼠睾丸Johnsen评分降低、生精细胞凋亡率升高;照后3 d,模型组和阳性药组小鼠体重降低,模型组、阳性药组、高剂量组睾丸Johnsen评分降低,各照射组生精细胞凋亡率均升高;照后7 d,各照射组睾丸指数下降,睾丸形态结构改变,Johnsen评分显著降低,生精细胞凋亡率进一步升高。与模型组相比,照后3、7 d,各给药组睾丸Johnsen评分显著升高,生精细胞凋亡率显著降低,睾丸病理改变较轻。结论 2.0 Gy 60Coγ射线可以造成小鼠睾丸形态结构改变以及增加生精细胞的凋亡,进而影响小鼠的生精功能,并随时间加重;而在照前预防性给予小鼠益气解毒方,有效地减轻了以上损伤。

Objective To observe the acute testicular injury in male Balb/c mice induced by 2.0 Gy 60Co γ rays and the preventive effect of Yiqi Jiedu Fang(Qi-boosting and Toxin-removing Decoction, YQJDF) administered before irradiation. Methods 200 male Balb/c mice were randomly divided into five groups: normal group, model group, positive drug group, and high-and low-dose YQJDF groups. Except for the normal group, the other mice were subjected to single-dose whole-body irradiation with 2.0 Gy 60Co γ rays. The mice in the intervention groups were given their corresponding drugs for 10 consecutive days before the irradiation, and at the same time the mice in the normal group and the model group were given deionized water. Mice tissues were collected 1 day before and on the 1st, 3rd, and 7th days after the irradiation, and the body weight, testicular parameters, morphological changes of the testis, and apoptosis of spermatogenic cells were measured. Results Compared with the normal group, there was no significant difference in the above mentioned parameters of mice between groups 1 day before the irradiation. However, 1 day after irradiation, mice in the model group had a decreased Johnsen score of testis and an increased apoptosis rate of spermatogenic cells. On the 3rd day after irradiation, the weight of mice in the model group and the positive drug group decreased, the Johnsen score of mice in the model group, the positive drug group and the high-dose YQJDF group decreased, and the apoptosis rate of spermatogenic cells in all groups subjected to irradiation increased. On the 7th day after irradiation, testicular parameters decreased in all the irradiated groups, testicular morphology and structure changed, Johnsen score decreased significantly, and the spermatogenic cell apoptosis rate increased further. Compared with the model group, on the 3rd and 7th day after the irradiation, the Johnsen score of all the intervention groups were notably higher, and the apoptosis rate of spermatogenic cells were significantly lower, and the testicular pathological changes were lighter. Conclusion 2.0 Gy 60Co γ ray can cause morphological and structural changes in mouse testis, and increase the apoptosis of spermatogenic cells, and in turn affect the spermatogenic function of mice. The preventive treatment with YQJDF before irradiation effectively reduced the above injuries.